Key features and details
- Mouse monoclonal [JE-10D4] to Vitellogenin
- Suitable for: ELISA, WB
- Reacts with: Zebrafish
- Isotype: IgG1
Product nameAnti-Vitellogenin antibody [JE-10D4]
See all Vitellogenin primary antibodies
DescriptionMouse monoclonal [JE-10D4] to Vitellogenin
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Zebrafish
Full length native vitellogenin purified from whole body homogenate of 17 beta estradiol treated zebrafish.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.08% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab36804 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
WB: 1/100 - 1/1000. Predicted molecular weight: 183 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceVitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
- Vg antibody
- VTG antibody
- VTG1 antibody
ab36804 has been referenced in 2 publications.
- Li Q et al. FOXL2 down-regulates vitellogenin expression at mature stage in Eriocheir sinensis. Biosci Rep 35:N/A (2015). PubMed: 26430246
- Fan X et al. Selective yolk deposition and mannose phosphorylation of lysosomal glycosidases in zebrafish. J Biol Chem 285:32946-53 (2010). WB ; Zebrafish . PubMed: 20729204