Overview

  • Product name

    Anti-Vitellogenin antibody [JE-2A6]
    See all Vitellogenin primary antibodies
  • Description

    Mouse monoclonal [JE-2A6] to Vitellogenin
  • Host species

    Mouse
  • Specificity

    Does not cross-react with vitellogenin from the other cyrinid species carp (Cyprinus carpio), fathead minnow (Pimephales promelas) or roach (Rutilus rutilus).
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Zebrafish
  • Immunogen

    Full length native vitellogenin purified from whole body homogenate of 17 beta estradiol treated zebrafish.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.08% Sodium azide
    Constituents: 1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    JE-2A6
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab36805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
WB
  • Application notes
    ELISA: 1/100 - 1/1000.
    Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
    WB: 1/100 - 1/1000. Predicted molecular weight: 183 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      Vitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
    • Cellular localization

      Secreted
    • Alternative names

      • Vg antibody
      • VTG antibody
      • VTG1 antibody

    Images

    • All lanes : Anti-Vitellogenin antibody [JE-2A6] (ab36805) at 1/200 dilution

      Lane 1 : Purified zebrafish vitellogenin at 2 µg
      Lane 2 : Zebrafish whole body homogenate, 17ß-estradiol treated at 10 µg
      Lane 3 : Zebrafish whole body homogenate at 10 µg
      Lane 4 : Carp plasma, 17ß-estradiol treated at 10 µl
      Lane 5 : Carp plasma at 10 µl
      Lane 6 : Fathead minnow plasma, 17ß-estradiol treated at 10 µl
      Lane 7 : Fathead minnow plasma at 10 µl
      Lane 8 : Roach plasma, 17ß-estradiol treated at 10 µl
      Lane 9 : Roach plasma at 10 µl

      Predicted band size: 183 kDa

    References

    ab36805 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab36805.
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