Key features and details
- Mouse monoclonal [JE-2A6] to Vitellogenin
- Suitable for: ELISA, WB
- Reacts with: Zebrafish
- Isotype: IgG2b
Product nameAnti-Vitellogenin antibody [JE-2A6]
See all Vitellogenin primary antibodies
DescriptionMouse monoclonal [JE-2A6] to Vitellogenin
SpecificityDoes not cross-react with vitellogenin from the other cyrinid species carp (Cyprinus carpio), fathead minnow (Pimephales promelas) or roach (Rutilus rutilus).
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Zebrafish
Full length native vitellogenin purified from whole body homogenate of 17 beta estradiol treated zebrafish.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.08% Sodium azide
Constituents: 1% BSA, 0.87% Sodium chloride, 1.64% Sodium phosphate
Concentration information loading...
PurityProtein G purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab36805 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Note: The antibody is dissolved in a buffer containing BSA, and is therefore not recommended to use for coating.
WB: 1/100 - 1/1000. Predicted molecular weight: 183 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceVitellogenin (Vg) is an egg yolk precursor protein expressed solely in female fish and is dormant in male fish, but in the presence of estrogenic EDCs, male fish can express the Vg gene but in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic Endocrine Disrupting Chemicals (EDCs).
- Vg antibody
- VTG antibody
- VTG1 antibody
All lanes : Anti-Vitellogenin antibody [JE-2A6] (ab36805) at 1/200 dilution
Lane 1 : Purified zebrafish vitellogenin at 2 µg
Lane 2 : Zebrafish whole body homogenate, 17ß-estradiol treated at 10 µg
Lane 3 : Zebrafish whole body homogenate at 10 µg
Lane 4 : Carp plasma, 17ß-estradiol treated at 10 µl
Lane 5 : Carp plasma at 10 µl
Lane 6 : Fathead minnow plasma, 17ß-estradiol treated at 10 µl
Lane 7 : Fathead minnow plasma at 10 µl
Lane 8 : Roach plasma, 17ß-estradiol treated at 10 µl
Lane 9 : Roach plasma at 10 µl
Predicted band size: 183 kDa
ab36805 has not yet been referenced specifically in any publications.