Anti-Vitronectin/S-Protein antibody [1G11E8] (ab201981)
Key features and details
- Mouse monoclonal [1G11E8] to Vitronectin/S-Protein
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Vitronectin/S-Protein antibody [1G11E8]
See all Vitronectin/S-Protein primary antibodies -
Description
Mouse monoclonal [1G11E8] to Vitronectin/S-Protein -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human Vitronectin/S-Protein aa 1-200. Recombinant fragment expressed in E coli
Database link: P04004 -
Positive control
- Human VTN (AA: 20-199) recombinant protein, VTN (AA: 20-199)-hIgGFc transfected HEK293 cell lysate, Hela cells, Human cervical cancer tissues, Human liver cancer tissues
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
1G11E8 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab201981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000.
|
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Flow Cyt |
1/200 - 1/400.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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IHC-P |
1/200 - 1/1000.
|
Notes |
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WB
1/500 - 1/2000. |
Flow Cyt
1/200 - 1/400. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
IHC-P
1/200 - 1/1000. |
Target
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Function
Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity. -
Tissue specificity
Plasma. -
Sequence similarities
Contains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain. -
Domain
The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin. -
Post-translational
modificationsSulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium. -
Cellular localization
Secreted, extracellular space. - Information by UniProt
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Database links
- Entrez Gene: 7448 Human
- Omim: 193190 Human
- SwissProt: P04004 Human
- Unigene: 2257 Human
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Alternative names
- Complement S Protein antibody
- Epibolin antibody
- S Protein antibody
see all
Images
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All lanes : Anti-Vitronectin/S-Protein antibody [1G11E8] (ab201981) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Vitronectin/S-Protein (AA: 20-199)-hIgGFc transfected HEK293 cell lysate -
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Vitronectin/S-Protein with ab201981 at 1/200 dilution with DAB staining
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Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Vitronectin/S-Protein with ab201981 at 1/200 dilution with DAB staining.
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Flow cytometric analysis of Hela cells labeling Vitronectin/S-Protein using ab201981 at 1/200 dilution (green) and negative control (red).
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Anti-Vitronectin/S-Protein antibody [1G11E8] (ab201981) at 1/500 dilution + Human Vitronectin/S-Protein (AA: 20-199) recombinant protein
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab201981 has been referenced in 1 publication.
- Fox CR & Parks GD Complement Inhibitors Vitronectin and Clusterin Are Recruited from Human Serum to the Surface of Coronavirus OC43-Infected Lung Cells through Antibody-Dependent Mechanisms. Viruses 14:N/A (2021). PubMed: 35062233