Key features and details
- Mouse monoclonal [1G11E8] to Vitronectin/S-Protein
- Suitable for: ELISA, WB, Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-Vitronectin/S-Protein antibody [1G11E8]
See all Vitronectin/S-Protein primary antibodies
DescriptionMouse monoclonal [1G11E8] to Vitronectin/S-Protein
Tested applicationsSuitable for: ELISA, WB, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human Vitronectin/S-Protein aa 20-199. Recombinant fragment expressed in E coli
D QESCKGRCTE GFNVDKKCQC DELCSYYQSC CTDYTAECKP QVTRGDVFTM PEDEYTVYDD GEEKNNATVH EQVGGPSLTS DLQAQSKGNP EQTPVLKPEE EAPAPEVGAS KPEGIDSRPE TLHPGRPQPP AEEELCSGKP FDAFTDLKNG SLFAFRGQYC YELDEKAVRP GYPKLIRDV
Database link: P04004
- Human VTN (AA: 20-199) recombinant protein, VTN (AA: 20-199)-hIgGFc transfected HEK293 cell lysate, Hela cells, Human cervical cancer tissues, Human liver cancer tissues
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab201981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000.|
|Flow Cyt||1/200 - 1/400.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|IHC-P||1/200 - 1/1000.|
FunctionVitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
Sequence similaritiesContains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain.
DomainThe SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
modificationsSulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium.
Cellular localizationSecreted, extracellular space.
- Information by UniProt
- Complement S Protein antibody
- Epibolin antibody
- S Protein antibody
All lanes : Anti-Vitronectin/S-Protein antibody [1G11E8] (ab201981) at 1/500 dilution
Lane 1 : HEK293 cell lysate
Lane 2 : Vitronectin/S-Protein (AA: 20-199)-hIgGFc transfected HEK293 cell lysate
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Vitronectin/S-Protein with ab201981 at 1/200 dilution with DAB staining
Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Vitronectin/S-Protein with ab201981 at 1/200 dilution with DAB staining.
Flow cytometric analysis of Hela cells labeling Vitronectin/S-Protein using ab201981 at 1/200 dilution (green) and negative control (red).
Anti-Vitronectin/S-Protein antibody [1G11E8] (ab201981) at 1/500 dilution + Human Vitronectin/S-Protein (AA: 20-199) recombinant protein
ab201981 has not yet been referenced specifically in any publications.