Overview

  • Product name

    Anti-Vitronectin/S-Protein antibody [1G11E8]
    See all Vitronectin/S-Protein primary antibodies
  • Description

    Mouse monoclonal [1G11E8] to Vitronectin/S-Protein
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, WB, Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human Vitronectin/S-Protein aa 20-199. Recombinant fragment expressed in E coli
    Sequence:

    D QESCKGRCTE GFNVDKKCQC DELCSYYQSC CTDYTAECKP QVTRGDVFTM PEDEYTVYDD GEEKNNATVH EQVGGPSLTS DLQAQSKGNP EQTPVLKPEE EAPAPEVGAS KPEGIDSRPE TLHPGRPQPP AEEELCSGKP FDAFTDLKNG SLFAFRGQYC YELDEKAVRP GYPKLIRDV


    Database link: P04004

  • Positive control

    • Human VTN (AA: 20-199) recombinant protein, VTN (AA: 20-199)-hIgGFc transfected HEK293 cell lysate, Hela cells, Human cervical cancer tissues, Human liver cancer tissues

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    1G11E8
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab201981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
WB 1/500 - 1/2000.
Flow Cyt 1/200 - 1/400.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/200 - 1/1000.

Target

  • Function

    Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • Tissue specificity

    Plasma.
  • Sequence similarities

    Contains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.
  • Domain

    The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • Post-translational
    modifications

    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization

    Secreted, extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Complement S Protein antibody
    • Epibolin antibody
    • S Protein antibody
    • S-protein antibody
    • Serum Spreading Factor antibody
    • Serum-spreading factor antibody
    • Somatomedin B antibody
    • Somatomedin-B antibody
    • V75 antibody
    • Vitronectin antibody
    • Vitronectin V10 subunit antibody
    • Vitronectin V65 subunit antibody
    • VN antibody
    • VNT antibody
    • VTN antibody
    • VTNC_HUMAN antibody
    see all

Images

  • All lanes : Anti-Vitronectin/S-Protein antibody [1G11E8] (ab201981) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : Vitronectin/S-Protein (AA: 20-199)-hIgGFc transfected HEK293 cell lysate
  • Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Vitronectin/S-Protein with ab201981 at 1/200 dilution with DAB staining

  • Immunohistochemical analysis of paraffin-embedded Human cervical cancer tissue labeling Vitronectin/S-Protein with ab201981 at 1/200 dilution with DAB staining.

  • Flow cytometric analysis of Hela cells labeling Vitronectin/S-Protein using ab201981 at 1/200 dilution (green) and negative control (red).

  • Anti-Vitronectin/S-Protein antibody [1G11E8] (ab201981) at 1/500 dilution + Human Vitronectin/S-Protein (AA: 20-199) recombinant protein

References

ab201981 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab201981.
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