Key features and details
- Rabbit polyclonal to Vitronectin/S-Protein
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Vitronectin/S-Protein antibody
See all Vitronectin/S-Protein primary antibodies
DescriptionRabbit polyclonal to Vitronectin/S-Protein
Reacts with both native monomeric and polymeric Vitronectin/S-Protein in various tissues and plasma.
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Human
Full length native protein (purified) corresponding to Human Vitronectin/S-Protein.
General notesThe concentration of this product is lot-specific.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.60
Constituents: 0.82% Sodium phosphate, 0.0292% EDTA, 0.58% Sodium chloride
Concentration information loading...
PurityProtein A purified
Purification notes>98% pure (SDS-PAGE).
Our Abpromise guarantee covers the use of ab20091 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 54 kDa. Dilution optimised using Chromogenic detection.|
FunctionVitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
Sequence similaritiesContains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain.
DomainThe SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
modificationsSulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium.
Cellular localizationSecreted, extracellular space.
- Information by UniProt
- Complement S Protein antibody
- Epibolin antibody
- S Protein antibody
ab20091 has been referenced in 3 publications.
- De Gaetano GV et al. The Streptococcus agalactiae cell wall-anchored protein PbsP mediates adhesion to and invasion of epithelial cells by exploiting the host vitronectin/av integrin axis. Mol Microbiol N/A:N/A (2018). PubMed: 30030946
- Paulsson M et al. Bacterial Outer Membrane Vesicles Induce Vitronectin Release Into the Bronchoalveolar Space Conferring Protection From Complement-Mediated Killing. Front Microbiol 9:1559 (2018). PubMed: 30061873
- Voss S et al. The choline-binding protein PspC of Streptococcus pneumoniae interacts with the C-terminal heparin-binding domain of vitronectin. J Biol Chem 288:15614-27 (2013). PubMed: 23603906