Overview

  • Product name

    Anti-Vitronectin/S-Protein antibody
    See all Vitronectin/S-Protein primary antibodies
  • Description

    Rabbit polyclonal to Vitronectin/S-Protein
  • Host species

    Rabbit
  • Specificity

    Reacts with both native monomeric and polymeric Vitronectin/S-Protein in various tissues and plasma.

  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Vitronectin/S-Protein.

  • General notes

    The concentration of this product is lot-specific.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 6.60
    Constituents: 0.82% Sodium phosphate, 0.0292% EDTA, 0.58% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    >98% pure (SDS-PAGE).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20091 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 54 kDa. Dilution optimised using Chromogenic detection.

Target

  • Function

    Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • Tissue specificity

    Plasma.
  • Sequence similarities

    Contains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.
  • Domain

    The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • Post-translational
    modifications

    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization

    Secreted, extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Complement S Protein antibody
    • Epibolin antibody
    • S Protein antibody
    • S-protein antibody
    • Serum Spreading Factor antibody
    • Serum-spreading factor antibody
    • Somatomedin B antibody
    • Somatomedin-B antibody
    • V75 antibody
    • Vitronectin antibody
    • Vitronectin V10 subunit antibody
    • Vitronectin V65 subunit antibody
    • VN antibody
    • VNT antibody
    • VTN antibody
    • VTNC_HUMAN antibody
    see all

References

This product has been referenced in:

  • De Gaetano GV  et al. The Streptococcus agalactiae cell wall-anchored protein PbsP mediates adhesion to and invasion of epithelial cells by exploiting the host vitronectin/av integrin axis. Mol Microbiol N/A:N/A (2018). Read more (PubMed: 30030946) »
  • Paulsson M  et al. Bacterial Outer Membrane Vesicles Induce Vitronectin Release Into the Bronchoalveolar Space Conferring Protection From Complement-Mediated Killing. Front Microbiol 9:1559 (2018). Read more (PubMed: 30061873) »
See all 3 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry. Sarah Mardle is off on maternity leave and in her absence I am dealing with her enquiries. I have heard back from the source of this antibody and I have determined that at present there are no specific working dilutions for ab20091 by ELISA. Unfortunately we do not sell a standard for this antibody. I have located some references which indicate that vitronectin is a plasma glycoprotein that circulates in the blood at a concentration range of 200 to 400 mg/ml constituting 0.2 to 0.5% of total plasma proteins. It is detected as a mixture of both 75 kDa and 65 kDa forms. The 65 kDa form is a product of proteolytic processing of the former which appears to be endogenously present. The references are listed below. Shaffer, M.C. et al., J. Lab. Clin. Med., 103, 783 (1984) Conlan, S.V. et al., Blood. , 72, 185 (1988) I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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