Key features and details
- Rabbit polyclonal to Vitronectin/S-Protein
- Suitable for: IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Vitronectin/S-Protein antibody
See all Vitronectin/S-Protein primary antibodies
DescriptionRabbit polyclonal to Vitronectin/S-Protein
Tested applicationsSuitable for: IHC-P, IPmore details
Species reactivityReacts with: Mouse, Human
- IHC-P: Human small intestine and placenta tissues. IP: NIH/3T3 whole cell lysate.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab235987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|IP||1/200 - 1/2000.|
FunctionVitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
Sequence similaritiesContains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain.
DomainThe SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
modificationsSulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium.
Cellular localizationSecreted, extracellular space.
- Information by UniProt
- Complement S Protein antibody
- Epibolin antibody
- S Protein antibody
Vitronectin/S-Protein was immunoprecipitated from 500 µg NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate with ab235987.
Lane 1: Rabbit control IgG IP in NIH/3T3 whole cell lysate.
Lane 2: ab235987 IP in NIH/3T3 whole cell lysate.
Lane 3: NIH/3T3 whole cell lysate 10 µg (Input).
For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
Paraffin-embedded human small intestine tissue stained for Vitronectin/S-Protein using ab235987 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human placenta tissue stained for Vitronectin/S-Protein using ab235987 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235987 has not yet been referenced specifically in any publications.