Anti-Vitronectin/S-Protein antibody (ab235987)

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Overview

  • Product name

    Anti-Vitronectin/S-Protein antibody
    See all Vitronectin/S-Protein primary antibodies

  • Description

    Rabbit polyclonal to Vitronectin/S-Protein

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment corresponding to Human Vitronectin/S-Protein aa 364-478.
    Sequence:

    SLAKKQRFRHRNRKGYRSQRGHSRGRNQNSRRPSRATWLSLFSSEESNLG ANNYDDYRMDWLVPATCEPIQSVFFFSGDKYYRVNLRTRRVDTVDPPYPR SIAQYWLGCPAPGHL


    Database link: P04004

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IHC-P: Human small intestine and placenta tissues. IP: NIH/3T3 whole cell lysate.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab235987 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200.
IP 1/200 - 1/2000.

Target

  • Function

    Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.

  • Tissue specificity

    Plasma.

  • Sequence similarities

    Contains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.

  • Domain

    The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.

  • Post-translational
    modifications

    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.

  • Cellular localization

    Secreted, extracellular space.
  • Information by UniProt

  • Database links

    • Alternative names

      • Complement S Protein antibody
      • Epibolin antibody
      • S Protein antibody
      • S-protein antibody
      • Serum Spreading Factor antibody
      • Serum-spreading factor antibody
      • Somatomedin B antibody
      • Somatomedin-B antibody
      • V75 antibody
      • Vitronectin antibody
      • Vitronectin V10 subunit antibody
      • Vitronectin V65 subunit antibody
      • VN antibody
      • VNT antibody
      • VTN antibody
      • VTNC_HUMAN antibody
      see all

    Images

    • Immunoprecipitation - Anti-Vitronectin/S-Protein antibody (ab235987)
      Immunoprecipitation - Anti-Vitronectin/S-Protein antibody (ab235987)

      Vitronectin/S-Protein was immunoprecipitated from 500 µg NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate with ab235987. 

      Lane 1: Rabbit control IgG IP in NIH/3T3 whole cell lysate.

      Lane 2: ab235987 IP in NIH/3T3 whole cell lysate.

      Lane 3: NIH/3T3 whole cell lysate 10 µg (Input).

      For western blotting, an HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody (ab235987)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody (ab235987)

      Paraffin-embedded human small intestine tissue stained for Vitronectin/S-Protein using ab235987 at 1/100 dilution in immunohistochemical analysis.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody (ab235987)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitronectin/S-Protein antibody (ab235987)

      Paraffin-embedded human placenta tissue stained for Vitronectin/S-Protein using ab235987 at 1/100 dilution in immunohistochemical analysis.

    References

    ab235987 has not yet been referenced specifically in any publications.

    Publishing research using ab235987? Please let us know so that we can cite the reference in this datasheet.

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