Recombinant
RabMAb

Recombinant Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139)

Overview

  • Product name

    Anti-Vitronectin/S-Protein antibody [EP873Y]
    See all Vitronectin/S-Protein primary antibodies
  • Description

    Rabbit monoclonal [EP873Y] to Vitronectin/S-Protein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Vitronectin/S-Protein aa 450-550. The exact sequence is proprietary.

  • Positive control

    • IHC-P: Human liver tissue. WB: Human serum tissue lysate
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP873Y
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab45139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 75 kDa.
IHC-P 1/100.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
      Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
    • Tissue specificity

      Plasma.
    • Sequence similarities

      Contains 4 hemopexin repeats.
      Contains 1 SMB (somatomedin-B) domain.
    • Domain

      The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
    • Post-translational
      modifications

      Sulfated on 2 tyrosine residues.
      N- and O-glycosylated.
      Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
      It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
      Phosphorylation sites are present in the extracellular medium.
    • Cellular localization

      Secreted, extracellular space.
    • Information by UniProt
    • Database links

    • Alternative names

      • Complement S Protein antibody
      • Epibolin antibody
      • S Protein antibody
      • S-protein antibody
      • Serum Spreading Factor antibody
      • Serum-spreading factor antibody
      • Somatomedin B antibody
      • Somatomedin-B antibody
      • V75 antibody
      • Vitronectin antibody
      • Vitronectin V10 subunit antibody
      • Vitronectin V65 subunit antibody
      • VN antibody
      • VNT antibody
      • VTN antibody
      • VTNC_HUMAN antibody
      see all

    Images

    • Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139) at 1/2000 dilution + Human serum tissue lysate at 20 µg with 5% NFDM/TBST

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

      Observed band size: 75 kDa
      why is the actual band size different from the predicted?


      Exposure time: 30 seconds
    • Ab45139 at a 1/100 dilution, staining human Vitronectin/S-Protein in human liver tissue by immunohistochemistry using paraffin embedded tissue.

    References

    This product has been referenced in:

    • Sinha M  et al. Direct conversion of injury-site myeloid cells to fibroblast-like cells of granulation tissue. Nat Commun 9:936 (2018). Read more (PubMed: 29507336) »
    • Hiratsuka S  et al. Hepato-entrained B220+CD11c+NK1.1+ cells regulate pre-metastatic niche formation in the lung. EMBO Mol Med 10:N/A (2018). Read more (PubMed: 29930175) »
    See all 9 Publications for this product

    Customer reviews and Q&As

    1-6 of 6 Abreviews or Q&A

    Question
    Answer

    Please find attached to this email the MSDS you requested for the following antibodies:
    ab3104
    ab18412
    ab45139
    ab68672

    A link to each of those MSDS will be shortly available from the product datasheets. This usually takes a few hours up to one day, delay that is necessary for the cache of the website to be regenerated.

    I hope these documents are useful to you. Do not hesitate to contact us again if you have further questions.

    I wish you good luck with your research work.

    Read More

    Answer

    Thank you for contacting us.

    I would like to inform you that we are currently dealing with your request.

    I will contact you again as soon as the requested documents are available.

    Thank you for your patience.

    Read More

    Question
    Answer

    Thank you for contacting Abcam.

    According to the details of our testing program we "offer a discount equal to one free antibody of your choice off a future purchase if you buy ab45139 now, test it in IHC-Fr and submit feedback to us in the form of an Abreview."

    I have not seen any order from NIH for this product. Please be sure to contact your purchase agent to be sure that this product has been ordered. If it has been ordered we may track this product if you could supply either the Abcam confirmation number for the order or the PO number used to order ab45139.



    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

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    Answer

    DISCOUNT CODE: ***********
    Expiration date: ************

    I am very pleased to hear you would like to accept our offer and test ab45139 in IHC-Frozen tissue.This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forIHC-Frozen tissueand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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    Question
    Answer

    Thank you very much for your interest in ab45139.

    To our knowledge, ab45139 has not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy ab45139 now, test it in IHC-Fr and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab45139 in IHC-Fr. I will then send a discount code. This code must be issued before purchasing ab45139 so please wait for my reply before ordering.

    2. Purchase ab45139 either by phone, fax, or online (www.abcam.com).

    3. Test it in IHC-Fr.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab45139 turns out to be unsuitable for IHC-Fr, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Read More

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab62769.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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