Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP873Y] to Vitronectin/S-Protein
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Product nameAnti-Vitronectin/S-Protein antibody [EP873Y]
See all Vitronectin/S-Protein primary antibodies
DescriptionRabbit monoclonal [EP873Y] to Vitronectin/S-Protein
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human Vitronectin/S-Protein aa 450-550. The exact sequence is proprietary.
- IHC-P: Human liver tissue. WB: Human serum tissue lysate
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab45139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 75 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/50 - 1/100.|
FunctionVitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
Sequence similaritiesContains 4 hemopexin repeats.
Contains 1 SMB (somatomedin-B) domain.
DomainThe SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
modificationsSulfated on 2 tyrosine residues.
N- and O-glycosylated.
Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
Phosphorylation sites are present in the extracellular medium.
Cellular localizationSecreted, extracellular space.
- Information by UniProt
- Complement S Protein antibody
- Epibolin antibody
- S Protein antibody
Anti-Vitronectin/S-Protein antibody [EP873Y] (ab45139) at 1/2000 dilution + Human serum tissue lysate at 20 µg with 5% NFDM/TBST
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Observed band size: 75 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Ab45139 at a 1/100 dilution, staining human Vitronectin/S-Protein in human liver tissue by immunohistochemistry using paraffin embedded tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab45139 has been referenced in 12 publications.
- Vicente-Munuera P et al. The topology of vitronectin: A complementary feature for neuroblastoma risk classification based on computer-aided detection. Int J Cancer 146:553-565 (2020). PubMed: 31173338
- Sheikh BN et al. Systematic Identification of Cell-Cell Communication Networks in the Developing Brain. iScience 21:273-287 (2019). PubMed: 31677479
- Burgos-Panadero R et al. Vitronectin as a molecular player of the tumor microenvironment in neuroblastoma. BMC Cancer 19:479 (2019). PubMed: 31117974
- Sinha M et al. Direct conversion of injury-site myeloid cells to fibroblast-like cells of granulation tissue. Nat Commun 9:936 (2018). PubMed: 29507336
- Hiratsuka S et al. Hepato-entrained B220+CD11c+NK1.1+ cells regulate pre-metastatic niche formation in the lung. EMBO Mol Med 10:N/A (2018). PubMed: 29930175