Overview

  • Product name

    Anti-Vitronectin/S-Protein antibody [VN58-1]
    See all Vitronectin/S-Protein primary antibodies
  • Description

    Mouse monoclonal [VN58-1] to Vitronectin/S-Protein
  • Host species

    Mouse
  • Specificity

    This antibody does not interfere with Vitronectin/S-Protein-mediated adhesion.
  • Tested applications

    Suitable for: ICC/IF, ELISA, WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Cow
  • Immunogen

    Full length protein corresponding to Human Vitronectin/S-Protein.

  • Epitope

    This antibody specifically reacts with an epitope in the region of amino acids 1-130 of human Vitronectin/S-Protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Ascites
  • Purification notes

    Purified from ascites.
  • Clonality

    Monoclonal
  • Clone number

    VN58-1
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab13413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
ELISA 1/3000. (with solid phase antigen)
WB Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 5 - 10 µg/ml.
IHC-Fr Use a concentration of 5 - 10 µg/ml.

Target

  • Function

    Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.
    Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
  • Tissue specificity

    Plasma.
  • Sequence similarities

    Contains 4 hemopexin repeats.
    Contains 1 SMB (somatomedin-B) domain.
  • Domain

    The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.
  • Post-translational
    modifications

    Sulfated on 2 tyrosine residues.
    N- and O-glycosylated.
    Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.
    It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.
    Phosphorylation sites are present in the extracellular medium.
  • Cellular localization

    Secreted, extracellular space.
  • Information by UniProt
  • Database links

  • Alternative names

    • Complement S Protein antibody
    • Epibolin antibody
    • S Protein antibody
    • S-protein antibody
    • Serum Spreading Factor antibody
    • Serum-spreading factor antibody
    • Somatomedin B antibody
    • Somatomedin-B antibody
    • V75 antibody
    • Vitronectin antibody
    • Vitronectin V10 subunit antibody
    • Vitronectin V65 subunit antibody
    • VN antibody
    • VNT antibody
    • VTN antibody
    • VTNC_HUMAN antibody
    see all

Images

  • This picture shows formalin-fixed paraffin embedded human skin stained with Vitronectin/S-Protein (1:100 - 30 minutes RT). The image was kindly supplied as part of the review submitted by Elizabeth Chlipala.

  • ICC/IF image of ab13413 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13413, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

  • Simsa R  et al. Effect of fluid dynamics on decellularization efficacy and mechanical properties of blood vessels. PLoS One 14:e0220743 (2019). Read more (PubMed: 31381614) »
  • Siney EJ  et al. Metalloproteinases ADAM10 and ADAM17 Mediate Migration and Differentiation in Glioblastoma Sphere-Forming Cells. Mol Neurobiol 54:3893-3905 (2017). Read more (PubMed: 27541285) »
See all 7 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Sample
Human Cell lysate - whole cell (Epithelial OVCA cell line)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
100 µg
Treatment
Perifosine 24 hr
Specification
Epithelial OVCA cell line
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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Submitted Aug 12 2016

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