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Hi, I've recently purchased the following products: ab13413 (mouse monoclonal VN58-1 to vitronectin), ab77811 (mouse monoclonal GMA-013 to plasminogen) and ab27710 (mouse monoclonal 04 to hemopexin). I`ve tried to use them in a WB assay utilizing HepG2 extract cells and the recommended dilutions but they doesn`t work. Is there a especific protocol that I have to follow? Yours faithfully,
Asked on Nov 21 2011
Thank you for contacting us. I am sorry that you are experiencing problems with these antibodies. In order to assist you as best I can could you please fill in the questionnaire I have attached to this email. This will allow me to understand more clearly the experiments that you have been performing and identify any advice I can give in order to achieve better results. It would be helpful if you did this for each antibody individually and if you could provide me with any images of the blots you have obtained this would also be of help. I look forward to hearing back from you.
Answered on Nov 21 2011