Overview

  • Product name
    Anti-VLDL Receptor / VLDL-R antibody
    See all VLDL Receptor / VLDL-R primary antibodies
  • Description
    Rabbit polyclonal to VLDL Receptor / VLDL-R
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Cow
  • Immunogen

    Synthetic peptide corresponding to Human VLDL Receptor/ VLDL-R aa 500-600 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab102057)

  • Positive control
    • This antibody gave a positive signal in Human ovary tissue lysate.
  • General notes

    Previously labelled as VLDL Receptor. 

Applications

Our Abpromise guarantee covers the use of ab92943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 96 kDa).

Target

  • Function
    Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation.
  • Tissue specificity
    Abundant in heart and skeletal muscle; also ovary and kidney; not in liver.
  • Involvement in disease
    Defects in VLDLR are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 1 (CMARQ1) [MIM:224050]; also known as dysequilibrium syndrome (DES) or non-progressive cerebellar disorder with mental retardation. CMARQ1 is a congenital, non-progressive cerebellar ataxia associated with disturbed equilibrium, delayed ambulation, mental retardation and cerebellar hypoplasia. Additional features include short stature, strabismus, pes planus and, rarely, seizures.
  • Sequence similarities
    Contains 3 EGF-like domains.
    Contains 8 LDL-receptor class A domains.
    Contains 6 LDL-receptor class B repeats.
  • Post-translational
    modifications
    Ubiquitinated at Lys-839 by MYLIP leading to degradation.
  • Cellular localization
    Membrane. Membrane > clathrin-coated pit.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ35024 antibody
    • Very low density lipoprotein receptor antibody
    • Very low-density lipoprotein receptor antibody
    • VLDL R antibody
    • VLDL receptor antibody
    • VLDL-R antibody
    • VLDLR antibody
    • VLDLR_HUMAN antibody
    • VLDLRCH antibody
    see all

Images

  • Anti-VLDL Receptor / VLDL-R antibody (ab92943) at 1 µg/ml + Human ovary tissue lysate - total protein (ab30222) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 96 kDa
    Observed band size: 130 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 24 kDa, 63 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 16 minutes


    The predicted molecular weight of VLDL Receptor / VLDL-R is 96 kDa (SwissProt), however we expect to observe a banding pattern at 130 kDa. VLDL Receptor / VLDL-R contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

References

This product has been referenced in:
  • Shin KC  et al. Macrophage VLDLR mediates obesity-induced insulin resistance with adipose tissue inflammation. Nat Commun 8:1087 (2017). Read more (PubMed: 29057873) »
  • Okoro EU  et al. A Subregion of Reelin Suppresses Lipoprotein-Induced Cholesterol Accumulation in Macrophages. PLoS One 10:e0136895 (2015). Read more (PubMed: 26317415) »
See all 4 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Answer

From your description it sounds like you are seeing something like what we show on the datasheet, a band at 130 kDa that we think is the receptor, and cross-reactivity with smaller proteins. The "predicted" size of 96 kDa is just that, a prediction based on the calculated weight of the primary amino acid sequence, but for many proteins, especially receptors modified post-translation, this is not the size that is observed in blots.

Proving that the 130 kDa band is the receptor is the difficult part. Knockdown of expression with siRNA is one validation control, and gene knockout is another, though it is often not a feasible approach. We do not have this kind of data for the majority of our antibodies.

Do you have any reason to believe that the 130 kDa band is not the receptor? As I mentioned in my previous e-mail, it seems like a big leap from an unmodified 96 kDa protein to 130 kDa, just from glycosylation, so I am unconvinced that the 130 band is the receptor. On the other hand, another customer sent us his ab75591 results for a human carcinoma, showing a band at about 150 kDa, which he considered good data. Here's a link to the review:

https://www.abcam.com/index.html?datasheet=75591&tab=abreviews&intabreviewid=19310

There is also a reference with mouse blot data for ab92943 though I am not able to open the article. Here is a link to the PubMed page:

http://www.ncbi.nlm.nih.gov/pubmed/22170052?dopt=Abstract

Please let me know how your re-test goes. If you can send an image file of the blot, that will help me understand what you are seeing.

Read More

Answer

Regarding a western blot protocol modification for the VLDLr antibody ab92943, this antibody does not require anything unusual, and I expect your protocol is fine if it is working for other antibodies. However, I recommend having a look at a recent customer review which discusses the effects on mouse tissue blot results of two different blocking solutions. Here is a link:

https://www.abcam.com/index.html?datasheet=92943&tab=abreviews&intabreviewid=32666

Can you tell me where you are seeing bands? The quality of the sample may be the more important considerationrather than the protocol. Did you use protease inhibitors when harvesting the placenta lysate and are other antibodies giving the expected results, assuming you are probing for other proteins too?

Our own result, a band at about 130 kDa for human ovary seems high, given that the precursor MW is calculated to be 96 kDa, but post-translation glycosylation was assumed to explain the migration to 130.

Read More
Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney, Testes, Cerebellum and spleen (see figure))
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel (Novex))
Loading amount
15 µg
Specification
Kidney, Testes, Cerebellum and spleen (see figure)
Blocking step
Milk, serum (see figure) and BSA tested as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 30 2012

Answer

I am very pleased to hear you would like to accept our offer and test ab92943 in mouse samples.

DISCOUNT CODE: 100%ABR-EB3EY
Expiration date: 1st Feb 2012

This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview formouse and include this code in the Additional Comments section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you very much for yourenquiry.

I am sorry to confirm thatour knowledge, ab92943 has not been tested in mouse. It is predicted to react, due to high sequence alignment of the immmunogen with the mouse VLDL Receptor sequence. However, I am sorry we cannot guarantee this without further testing.

Therefore, I can offer a discount off a future purchase if you buy ab92943 now, test it inmouse and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab92943 in mouse. I will then send a discount code. This code must be issued before purchasing ab92943 so please wait for my reply before ordering.

2. Purchase ab92943 either by phone, fax, or online (www.abcam.com).

3. Test it in mouse.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab92943 turns out to be unsuitable for mouse, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us.


As we had spoken of I have instructed our products team to add the blocking peptide for this ab92943. The product number is ab102057 and can be found at the following link: https://www.abcam.com/VLDL-Receptor-peptide-ab102057.html I have also attached our protocol for use of the blocking peptide in WB.


As we had also discussed, use of a casein based blocking buffer could help as well. I would recommend using ab64234 (https://www.abcam.com/Background-Reducing-Buffer-ab64234.html) in lieu of milk or bsa as a blocking solution.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your enquiry.

This product has only been tested withHuman samples usingwestern blot (WB). The cross reactivity with rat is solely based on homology sequence (94% homology) with the immunogen.Therefore, I am afraid that we will not be able toguarantee that you will beable to obtain good results if usedonrat samples.

We do not offer free samples but I can offer a discount off a future purchase if you buy ab92943, test it in rat using WB and submit feedback to us in the form of an Abreview. Your results may be either positive or negative, and the discount would be the value of one free primary antibody of your choice. If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab92943 is western blot. I will then send you a discount code. This code must be issued before purchasing the antibodyso please wait for my reply before ordering.

2. Purchase ab92943 through your local sub-dealer.

3. Test it.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order and mention the discount code to the sub-dealer. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab92943 turns out to be unsuitable for western blot, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

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