Overview

  • Product name

    Anti-VLDL Receptor/VLDL-R antibody
    See all VLDL Receptor/VLDL-R primary antibodies
  • Description

    Rabbit polyclonal to VLDL Receptor/VLDL-R
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VLDL Receptor/VLDL-R aa 555-605 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Sequence:

    LREPAS IAVDPLSGFV YWSDWGEPAK IEKAGMNGFD RRPLVTADIQ WPNGI


    Database link: P98155

  • Positive control

    • Rat heart and kidney lysates; mouse embryo tissue.
  • General notes

    Previously labelled as VLDL Receptor. 

Applications

Our Abpromise guarantee covers the use of ab203271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 96 kDa.
IHC-P 1/100 - 1/500.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200.

Flow Cyt 1/20 - 1/100.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Binds VLDL and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. Binding to Reelin induces tyrosine phosphorylation of Dab1 and modulation of Tau phosphorylation.
  • Tissue specificity

    Abundant in heart and skeletal muscle; also ovary and kidney; not in liver.
  • Involvement in disease

    Defects in VLDLR are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 1 (CMARQ1) [MIM:224050]; also known as dysequilibrium syndrome (DES) or non-progressive cerebellar disorder with mental retardation. CMARQ1 is a congenital, non-progressive cerebellar ataxia associated with disturbed equilibrium, delayed ambulation, mental retardation and cerebellar hypoplasia. Additional features include short stature, strabismus, pes planus and, rarely, seizures.
  • Sequence similarities

    Contains 3 EGF-like domains.
    Contains 8 LDL-receptor class A domains.
    Contains 6 LDL-receptor class B repeats.
  • Post-translational
    modifications

    Ubiquitinated at Lys-839 by MYLIP leading to degradation.
  • Cellular localization

    Membrane. Membrane > clathrin-coated pit.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ35024 antibody
    • Very low density lipoprotein receptor antibody
    • Very low-density lipoprotein receptor antibody
    • VLDL R antibody
    • VLDL receptor antibody
    • VLDL-R antibody
    • VLDLR antibody
    • VLDLR_HUMAN antibody
    • VLDLRCH antibody
    see all

Images

  • All lanes : Anti-VLDL Receptor/VLDL-R antibody (ab203271) at 1/200 dilution

    Lane 1 : Rat heart lysate
    Lane 2 : Rat kidney lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG Antibody (H+L), HRP at 1/3000 dilution

    Developed using the ECL technique.

    Predicted band size: 96 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse embryo tissue labeling VLDL Receptor/VLDL-R with ab203271 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

  • HEK 239T cells probed with ab203271 at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).

References

ab203271 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Age P0 brain (Subventricular zone))
Permeabilization
Yes - 0.05% Triton
Specification
Age P0 brain (Subventricular zone)
Blocking step
TNB Blocking Buffer from Perkin Elmer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Apr 23 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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