Overview

  • Product name

  • Description

    Rabbit polyclonal to VMA21
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Cow, Zebrafish, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human VMA21 aa 51-101. The exact sequence is proprietary.
    Database link: Q3ZAQ7

  • Positive control

    • WB: HeLa, HEK-293T, jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab242099 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 11 kDa.
IP Use at an assay dependent concentration.

20 μl/mg lysate

 

Target

  • Relevance

    VMA21 is the homolog of S. cerevisiae's vacuolar H+-ATPase and is essential for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum.
  • Cellular localization

    Endoplasmic reticulum
  • Database links

  • Alternative names

    • MEAX antibody
    • Myopathy with excessive autophagy protein antibody
    • Vacuolar ATPase assembly integral membrane protein VMA21 antibody
    • VMA21 antibody
    • XMEA antibody
    see all

Images

  • VMA21 IP in HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab242099 at 20 µL per reaction. Western blot was performed on the immunoprecipitate using ab242099 at 1/100 dilution.

    Lane 1: ab242099 IP in HEK-293T whole cell lysate.
    lane 2: Control IgG in HEK-293T whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

    Lysates prepared in NETN buffer.

     

     

  • All lanes : Anti-VMA21 antibody (ab242099) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 15 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 11 kDa


    Exposure time: 30 seconds


    Lysates prepared in NETN buffer.

References

ab242099 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab242099.
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