Product nameAnti-VMA21 antibody
See all VMA21 primary antibodies
DescriptionRabbit polyclonal to VMA21
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig
- HepG2 cell lysate Human Kidney Tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab62591 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 µg/ml. Predicted molecular weight: 11 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
Titre using peptide based assay: 1:1562500.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
RelevanceVMA21 is the homolog of S. cerevisiae's vacuolar H+-ATPase and is essential for the assembly of the V0 complex of the vacuolar ATPase (V-ATPase) in the endoplasmic reticulum.
Cellular localizationEndoplasmic reticulum
- MEAX antibody
- Myopathy with excessive autophagy protein antibody
- Vacuolar ATPase assembly integral membrane protein VMA21 antibody
All lanes : Anti-VMA21 antibody (ab62591) at 5 µg/ml
Lane 1 : Molecular Weight Marker
Lane 2 : HepG2 Cell lysate at 10 µg
All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDa
Additional bands at: 30 kDa (possible cross reactivity)
Antibodies diluted in 5% skim milk / PBS buffer. 15% Gel used.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling VMA21 with ab62591 at 4-8µg/ml. Arrows indicate positively labelled epitehlial cells of the renal tubule. Magnification: 400X.
ab62591 has not yet been referenced specifically in any publications.