Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6476] to VMAT1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-VMAT1 antibody [EPR6476]
See all VMAT1 primary antibodies
DescriptionRabbit monoclonal [EPR6476] to VMAT1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human VMAT1. The exact sequence is proprietary.
Database link: P54219
- BxPC-3, PC-12 and A549 cell lysates, A549 cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab168347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 56 kDa.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionInvolved in the vesicular transport of biogenic amines.
Sequence similaritiesBelongs to the major facilitator superfamily. Vesicular transporter family.
Cellular localizationCytoplasmic vesicle membrane.
- Information by UniProt
- 4832416I10Rik antibody
- Adrenal chromaffin granule amine transporter; CGAT antibody
- CGAT antibody
Flow Cytometrical analysis of permeabilized A549 cells with ab168347 antibody at a dilution of 1/100 (red) or a rabbit IgG negative (green).
All lanes : Anti-VMAT1 antibody [EPR6476] (ab168347) at 1/1000 dilution
Lane 1 : BxPC-3 cell lysate
Lane 2 : PC-12 cell lysate
Lane 3 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 56 kDa
ab168347 has been referenced in 3 publications.
- Wu Q et al. Dynamin 1 Restrains Vesicular Release to a Subquantal Mode In Mammalian Adrenal Chromaffin Cells. J Neurosci 39:199-211 (2019). PubMed: 30381405
- Sato DX et al. Human-specific mutations in VMAT1 confer functional changes and multi-directional evolution in the regulation of monoamine circuits. BMC Evol Biol 19:220 (2019). PubMed: 31791232
- Zhang Q et al. Differential Co-release of Two Neurotransmitters from a Vesicle Fusion Pore in Mammalian Adrenal Chromaffin Cells. Neuron 102:173-183.e4 (2019). PubMed: 30773347