Anti-VMAT2 antibody (ab191121)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-VMAT2 antibody
See all VMAT2 primary antibodies -
Description
Rabbit polyclonal to VMAT2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Rat VMAT2 aa 450 to the C-terminus. The exact sequence is proprietary.
Database link: Q01827 -
Positive control
- Human fetal brain, rat brain and mouse brain lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 99% PBS, BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab191121 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/200. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
Target
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Function
Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis. -
Sequence similarities
Belongs to the major facilitator superfamily. Vesicular transporter family. -
Cellular localization
Cytoplasmic vesicle membrane. - Information by UniProt
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Database links
- Entrez Gene: 6571 Human
- Entrez Gene: 214084 Mouse
- Entrez Gene: 25549 Rat
- Omim: 193001 Human
- SwissProt: Q05940 Human
- SwissProt: Q8BRU6 Mouse
- SwissProt: Q01827 Rat
- Unigene: 596992 Human
see all -
Alternative names
- 1110037L13Rik antibody
- 9330105E13 antibody
- MGC120477 antibody
see all
Images
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Western blot - Anti-VMAT2 antibody (ab191121)Anti-VMAT2 antibody (ab191121) at 1/1000 dilution + Human fetal brain lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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Western blot - Anti-VMAT2 antibody (ab191121)Anti-VMAT2 antibody (ab191121) at 1/200 dilution + Rat brain lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
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Western blot - Anti-VMAT2 antibody (ab191121)Anti-VMAT2 antibody (ab191121) at 1/1000 dilution + Mouse brain lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa
Observed band size: 56 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Protocols
References
This product has been referenced in:
- Nyarko JNK et al. Glycosylation States of Pre- and Post-synaptic Markers of 5-HT Neurons Differ With Sex and 5-HTTLPR Genotype in Cortical Autopsy Samples. Front Neurosci 12:545 (2018). Read more (PubMed: 30147642) »
