Anti-VNN1/Vanin-1 antibody (ab231056)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-VNN1/Vanin-1 antibody
See all VNN1/Vanin-1 primary antibodies -
Description
Rabbit polyclonal to VNN1/Vanin-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse
-
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Rat VNN1/Vanin-1 aa 22-328. (Expressed in E.coli).
Sequence:SSLDTFLAAVYEHAVILPKVTLLPVSHSEALALMNQNLDLLEGAILSAAK QGAHIIVTPEDGIYGVQFTRDTIYPYLEDIPDPQVNWIPCDNPERFGSTP VQERLSCLAKNNSIYVVANMGDKKPCNTSDSHCPPDGRFQYNTDVVFDSR GKLVARYHKQNLFMGEEQFNAPPEPEVVTFDTPFGKFGIFTCFDILFHDP AVTLVTEFQVDTILFPTAWMDVLPHLAAIEFHSAWAIGMGVNFLAANLHI PLRRMTGSGIYAPDSPRAFHYDRKTQEGKLLLAQLDSHPSHSPVNWTSYA SSVEAPP
Database link: Q4KLZ0 -
Positive control
- IHC-P: Rat liver tissue. WB: Rat serum; Recombinant rat VNN1/Vanin-1 protein.
-
General notes
This product was previously labelled as VNN1
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab231056 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab231056 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 57 kDa. | |
| IHC-P | Use a concentration of 5 - 20 µg/ml. |
Target
-
Function
Amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. -
Tissue specificity
Widely expressed with higher expression in spleen, kidney and blood. Overexpressed in lesional psoriatic skin. -
Sequence similarities
Belongs to the CN hydrolase family. BTD/VNN subfamily.
Contains 1 CN hydrolase domain. -
Cellular localization
Cell membrane. - Information by UniProt
-
Database links
- Entrez Gene: 22361 Mouse
- Entrez Gene: 29142 Rat
- SwissProt: Q9Z0K8 Mouse
- Unigene: 27154 Mouse
-
Alternative names
- HDLCQ8 antibody
- High density lipoprotein cholesterol level quantitative trait locus 8, included antibody
- Pantetheinase antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VNN1/Vanin-1 antibody (ab231056)Formalin-fixed, paraffin-embedded rat liver tissue stained for VNN1/Vanin-1 using ab231056 at 20 µg/ml in immunohistochemical analysis, followed by DAB staining.
-
Western blot - Anti-VNN1/Vanin-1 antibody (ab231056)Anti-VNN1/Vanin-1 antibody (ab231056) at 1 µg/ml + Rat serum
Predicted band size: 57 kDa -
Western blot - Anti-VNN1/Vanin-1 antibody (ab231056)Anti-VNN1/Vanin-1 antibody (ab231056) at 2 µg/ml + Recombinant rat VNN1/Vanin-1 protein
Predicted band size: 57 kDa
Datasheets and documents
References
ab231056 has not yet been referenced specifically in any publications.
