Overview

  • Product name
    Anti-Von Willebrand Factor antibody [36B11]
    See all Von Willebrand Factor primary antibodies
  • Description
    Mouse monoclonal [36B11] to Von Willebrand Factor
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-Fr, IHC-Pmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fusion protein corresponding to the A2 domain of the von Willebrand factor.

  • Positive control
    • Human tonsil tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab49706 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/100 - 1/200. Acetone fixation recommended.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
    • Tissue specificity
      Plasma.
    • Involvement in disease
      Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
    • Sequence similarities
      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 4 TIL (trypsin inhibitory-like) domains.
      Contains 3 VWFA domains.
      Contains 3 VWFC domains.
      Contains 4 VWFD domains.
    • Domain
      The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
    • Post-translational
      modifications
      All cysteine residues are involved in intrachain or interchain disulfide bonds.
      N- and O-glycosylated.
    • Cellular localization
      Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • Coagulation factor VIII antibody
      • Coagulation factor VIII VWF antibody
      • F8VWF antibody
      • Factor VIII related antigen antibody
      • von Willebrand antigen 2 antibody
      • von Willebrand antigen II antibody
      • Von Willebrand disease antibody
      • VWD antibody
      • vWF antibody
      • VWF_HUMAN antibody
      see all

    References

    This product has been referenced in:
    • Vishnubalaji R  et al. In vitro differentiation of human skin-derived multipotent stromal cells into putative endothelial-like cells. BMC Dev Biol 12:7 (2012). ICC/IF ; Human . Read more (PubMed: 22280443) »
    • Allameh A  et al. In Vivo Vascularization of Endothelial Cells Derived from Bone Marrow Mesenchymal Stem Cells in SCID Mouse Model. Cell J 18:179-88 (0). IHC . Read more (PubMed: 27540522) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Vielen Dank für Ihren Anruf.

    Nachdem ich wie versprochen alle unsere Von Willebrand Faktor- Antikörperdaraufhin untersucht habe, ob sie möglicherweise die Bindung von Faktor VIII inhibieren können, weiß ich nicht, ob ich wirklich gute Nachrichten für Sie habe:

    Von denen, die gegen eine bestimmte Sequenz von vWF gerichtet sind, wären eventuell ab96340 oder ab93708 die aussichtsreichsten Kandidaten. Die Immunogene liegen zwischen Aminosäuren 1100 und 1200, was noch am nächsten an der D3-Domäne (Aminosäuren 856-1011/1074) gelegen ist. Ob dies allerdings für die Inhibition der Bindung ausreicht, ist nicht geklärt und müsste getestet werden.

    ab96340: https://www.abcam.com/index.html?datasheet=96340 (or use the following: https://www.abcam.com/index.html?datasheet=96340).

    ab93708: https://www.abcam.com/index.html?datasheet=93708 (or use the following: https://www.abcam.com/index.html?datasheet=93708).

    (Folgende Antikörper sind gegen weiter entfernte Regionen gerichtet, und sind daher wohl nicht geeignet: ab47160, ab47129, ab47139, ab47151, ab49706)

    Daneben haben wir noch einige Antikörper, die gegen das Volllängenprotein generiert wurden. Da ein Epitopmapping routinemäβig nicht durchgeführt wird, kann man nur experimentell bestimmen, wo das/die Epitope liegen bzw. ob eine Faktor VIII-Bindung inhibiert werden kann. Hier würde ich empfehlen, Antikörper zu testen, die wir für die Anwendung im ELISA garantieren können:

    ab11713: https://www.abcam.com/index.html?datasheet=11713 (or use the following: https://www.abcam.com/index.html?datasheet=11713).

    ab68545: https://www.abcam.com/index.html?datasheet=68545 (or use the following: https://www.abcam.com/index.html?datasheet=68545).

    ab115771: https://www.abcam.com/index.html?datasheet=115771 (or use the following: https://www.abcam.com/index.html?datasheet=115771).

    ab61292:https://www.abcam.com/index.html?datasheet=61292 (or use the following: https://www.abcam.com/index.html?datasheet=61292).

    Bei allen anderen wäre es reine Spekulation, ob Sie im ELISA funktionieren UND die Bindung blockieren.

    Falls Sie trotz all dieser Unwägbarkeiten einen dieser Antikörper in Ihren Versuchen testen möchten, kann ich Ihnenden beim letzten Mal erwähnten Testrabatt anbieten. Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link noch einmal nachlesen: https://www.abcam.com/collaborationdiscount

    Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

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    Answer

    Thank you for contacting us.

    I am looking into adding the VW33-5 clone to our catalogue, and will e-mail you when I find out if it is available. The other clone, N10, is described in the paper at the following link. I think this must be the paper you mentioned.

    http://www.ncbi.nlm.nih.gov/pubmed/16934083

    I have not been able to find a source, and I suspect it is not commercially available. I suggest contacting the authors of the paper. They may be kind enough to send you a sample.

    Read More

    Answer

    Thank you for contacting Abcam.

    I wish to apologize for the delay in getting this information about ananti-Von Willebrand antibody which is specific to human and has less than 10% mouse cross reactivity.

    I have gone through each of the products in our catalog and spoken to the labs or suppliers which create them. No product currently has a level of cross reactivity with mouse which is at or below the 10% threshold.

    Of the products we currently have, ab47906, which has been created from a recombinant fusion protein corresponding to the A2 domain of von Willebrand factor, has the lowest homology with the mouse protein at 75%. We would not recommend this as a viable product for detecting mouse von Willebrand. To our knowledge this however has not been tested.

    I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

    Read More

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