Key features and details
- Mouse monoclonal [3E2D10] to Von Willebrand Factor
- Suitable for: Flow Cyt, WB, IP, ICC/IF, IHC-P, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-Von Willebrand Factor antibody [3E2D10]
See all Von Willebrand Factor primary antibodies
DescriptionMouse monoclonal [3E2D10] to Von Willebrand Factor
Tested applicationsSuitable for: Flow Cyt, WB, IP, ICC/IF, IHC-P, IHC-Frmore details
Species reactivityReacts with: Human
- HUVEC cells or Tonsil
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Concentration information loading...
PurityProtein G purified
Purification notesPurified from Bioreactor Concentrate
Light chain typekappa
Our Abpromise guarantee covers the use of ab194405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.5-1µl for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 309 kDa.|
|IP||Use at 0.25-500 µg/mg of lysate.|
|ICC/IF||Use a concentration of 0.5 - 1 µg/ml.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
use 10mM Tris with 1mM EDTA, pH 9.0 for antigen retrieval.
|IHC-Fr||Use a concentration of 0.5 - 1 µg/ml.|
FunctionImportant in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
Involvement in diseaseDefects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
Sequence similaritiesContains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains.
DomainThe von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
modificationsAll cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated.
Cellular localizationSecreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
- Information by UniProt
- Coagulation factor VIII antibody
- Coagulation factor VIII VWF antibody
- F8VWF antibody
ab194405 has been referenced in 4 publications.
- Ito Y et al. Turbulence Activates Platelet Biogenesis to Enable Clinical Scale Ex Vivo Production. Cell 174:636-648.e18 (2018). PubMed: 30017246
- Klotz BJ et al. Engineering of a complex bone tissue model with endothelialised channels and capillary-like networks. Eur Cell Mater 35:335-348 (2018). PubMed: 29873804
- Garbuzova-Davis S et al. Endothelial and Astrocytic Support by Human Bone Marrow Stem Cell Grafts into Symptomatic ALS Mice towards Blood-Spinal Cord Barrier Repair. Sci Rep 7:884 (2017). IHC ; Mouse . PubMed: 28408761
- Yue X et al. Endothelial lipase is upregulated by interleukin-6 partly via the p38 MAPK and p65 NF-?B signaling pathways. Mol Med Rep 14:1979-85 (2016). Human . PubMed: 27430252