Anti-Von Willebrand Factor antibody (ab11713)
Key features and details
- Sheep polyclonal to Von Willebrand Factor
- Suitable for: Immunodiffusion, ELISA, ICC/IF, IHC-Fr, Flow Cyt
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Von Willebrand Factor antibody
See all Von Willebrand Factor primary antibodies -
Description
Sheep polyclonal to Von Willebrand Factor -
Host species
Sheep -
Tested applications
Suitable for: Immunodiffusion, ELISA, ICC/IF, IHC-Fr, Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human, Pig -
Immunogen
Full length native protein (purified) corresponding to Human Von Willebrand Factor. Purified human von Willebrand factor prepared from citrated human plasma
Database link: P04275 -
General notes
This product should be stored undiluted. Storage in frost free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: Glycine buffered saline, 0.1% EACA, 0.01% Benzamidine, 0.0292% EDTA -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Purification notes
Purified IgG was prepared from serum by ion exchange chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11713 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Immunodiffusion |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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ICC/IF | (2) |
Use at an assay dependent concentration.
|
IHC-Fr | (6) |
Use at an assay dependent concentration.
|
Flow Cyt |
Use at an assay dependent concentration.
ab37385 - Sheep polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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Immunodiffusion
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab37385 - Sheep polyclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
Domain
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. -
Post-translational
modificationsAll cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated. -
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules. - Information by UniProt
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Database links
- Entrez Gene: 7450 Human
- Entrez Gene: 22371 Mouse
- Omim: 613160 Human
- SwissProt: P04275 Human
- SwissProt: Q8CIZ8 Mouse
- Unigene: 440848 Human
- Unigene: 22339 Mouse
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Alternative names
- Coagulation factor VIII antibody
- Coagulation factor VIII VWF antibody
- F8VWF antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (105)
ab11713 has been referenced in 105 publications.
- Galley JC et al. Angiotensin II augments renal vascular smooth muscle soluble GC expression via an AT1 receptor-forkhead box subclass O transcription factor signalling axis. Br J Pharmacol 179:2490-2504 (2022). PubMed: 33963547
- Li B et al. RNA N6-methyladenosine modulates endothelial atherogenic responses to disturbed flow in mice. Elife 11:N/A (2022). PubMed: 35001873
- Huisman C et al. Critical changes in hypothalamic gene networks in response to pancreatic cancer as found by single-cell RNA sequencing. Mol Metab 58:101441 (2022). PubMed: 35031523
- Desilles JP et al. Impact of COVID-19 on thrombus composition and response to thrombolysis: Insights from a monocentric cohort population of COVID-19 patients with acute ischemic stroke. J Thromb Haemost 20:919-928 (2022). PubMed: 35032088
- Dosunmu-Ogunbi A et al. Endothelial superoxide dismutase 2 is decreased in sickle cell disease and regulates fibronectin processing. Function (Oxf) 3:zqac005 (2022). PubMed: 35274104