Immunohistochemistry (Frozen sections) abreview for Anti-Von Willebrand Factor antibody

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Pig Tissue sections (Heart)
Specification
Heart
Fixative
Acetone
Permeabilization
Yes - 0.3 % Triton in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C

Other product details

Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Donkey pAb to Sheep FITC
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/1000

Additional data

Additional Notes
The green color indicates the staining of VWF. The blue color indicates staining of nuclei using DAPI.

Abcam user community

Verified customer

Submitted Dec 15 2011

Sign up