Flow Cytometry abreview for Anti-Von Willebrand Factor antibody

Inconclusive
Abreviews
Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.2% saponin
Sample
Mouse Cell (skin)
Specification
skin
Preparation
Cell harvesting/tissue preparation method: Wilson L, Fathke C, Isik F. Tissue dispersion and flow cytometry for the cellular analysis of wound healing. BioTechniques 2002: 32: 548-551.
Sample buffer: 2%BSA/PBS

Other product details

Incubation time
30 minute(s)
Dilution
1/200

Secondary antibody

Dilution
1/1000

Additional data

Additional Notes
1/200, 1/400, 1/800, 1/1000, and 1/2000 dilutions tested, all negative results
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.

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Submitted May 14 2014

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