This antibody stained rat smooth muscle cells, making it non-specific for endothelial cells. Attached is an image of A7r5 cells using the Abcam primary anti-von willebrand factor antibody with a high level of nuclear staining. I also imaged a no-primary control which does not show any significant amount of staining, indicating this is not a non-specific secondary antibody problem.
We would like to thank the reviewer for submitting this Abreview for this product. As the product had not yet been tested in this species, we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. The customer has received value off a future purchase for performing these tests. We wish to thank them for participating in our AbTrial program.
Submitted May 02 2016
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