Anti-Von Willebrand Factor antibody (ab6994)
Key features and details
- Rabbit polyclonal to Von Willebrand Factor
- Suitable for: WB, ICC/IF, IHC-Fr, Flow Cyt, IHC-P, IHC-FoFr, IHC-FrFl
- Reacts with: Rat, Sheep, Horse, Guinea pig, Cow, Dog, Human, Pig
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Von Willebrand Factor antibody
See all Von Willebrand Factor primary antibodies -
Description
Rabbit polyclonal to Von Willebrand Factor -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Fr, Flow Cyt, IHC-P, IHC-FoFr, IHC-FrFlmore details -
Species reactivity
Reacts with: Rat, Sheep, Horse, Guinea pig, Cow, Dog, Human, Pig
Does not react with: Chicken -
Immunogen
Full length native protein (purified) corresponding to Human Von Willebrand Factor. Purified from plasma.
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Positive control
- IHC-P: Human kidney tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other rabbit serum proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (10) |
Use at an assay dependent concentration.
|
| ICC/IF | (8) |
Use at an assay dependent concentration.
|
| IHC-Fr | (14) |
Use at an assay dependent concentration.
|
| Flow Cyt | (1) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
| IHC-P | (22) |
1/200 - 1/400. for IF and 1/1000-1/2000 for ABC methods with HRP conjugates. Perform enzymatic antigen retrieval with 0.1% pronase for 10 min at 35 °C before commencing with IHC protocol.
Indirect Immunofluorescence: minimum working dilution of 1:200 was determined using FFPE sections of human tongue with FITC-conjugated secondary.
Indirect Immunoperoxidase Labeling: minimum working dilution of 1:800 was determined using FFPE sections of human tongue with biotinylated secondary and signal amplification.
|
| IHC-FoFr | (3) |
Use at an assay dependent concentration. PubMed: 19622235
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| IHC-FrFl | (2) |
Use at an assay dependent concentration.
(see Abreview) |
| Notes |
|---|
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WB
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P
1/200 - 1/400. for IF and 1/1000-1/2000 for ABC methods with HRP conjugates. Perform enzymatic antigen retrieval with 0.1% pronase for 10 min at 35 °C before commencing with IHC protocol. Indirect Immunofluorescence: minimum working dilution of 1:200 was determined using FFPE sections of human tongue with FITC-conjugated secondary. Indirect Immunoperoxidase Labeling: minimum working dilution of 1:800 was determined using FFPE sections of human tongue with biotinylated secondary and signal amplification. |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 19622235 |
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IHC-FrFl
Use at an assay dependent concentration. (see Abreview) |
Target
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Function
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
Domain
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. -
Post-translational
modificationsAll cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated. -
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules. - Information by UniProt
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Database links
- Entrez Gene: 7450 Human
- Entrez Gene: 116669 Rat
- Omim: 613160 Human
- SwissProt: P04275 Human
- SwissProt: Q62935 Rat
- Unigene: 440848 Human
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Alternative names
- Coagulation factor VIII antibody
- Coagulation factor VIII VWF antibody
- F8VWF antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (ab6994)
Immunohistochemical analysis of Formalin fixed paraffin-embedded sections human kidney tissue labeling Von Willebrand Factor with ab6994 at 1/2000.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (ab6994)Immunohistochemical analysis of Formalin fixed paraffin-embedded sections human kidney tissue labeling Von Willebrand Factor with ab6994 at 1/2000.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (385)
ab6994 has been referenced in 385 publications.
- Hørlyck S et al. ATP induces contraction of cultured brain capillary pericytes via activation of P2Y-type purinergic receptors. Am J Physiol Heart Circ Physiol 320:H699-H712 (2021). PubMed: 33306443
- Huang S et al. Co-expression of tissue kallikrein 1 and tissue inhibitor of matrix metalloproteinase 1 improves myocardial ischemia-reperfusion injury by promoting angiogenesis and inhibiting oxidative stress. Mol Med Rep 23:N/A (2021). PubMed: 33355364
- Rosendorf J et al. Reinforcement of Colonic Anastomosis with Improved Ultrafine Nanofibrous Patch: Experiment on Pig. Biomedicines 9:N/A (2021). PubMed: 33494257
- Shiroma ME et al. Pretreatment with melatonin improves ovarian tissue cryopreservation for transplantation. Reprod Biol Endocrinol 19:17 (2021). PubMed: 33536029
- Liu MM et al. Diammonium glycyrrhizinate lipid ligand ameliorates lipopolysaccharide-induced acute lung injury by modulating vascular endothelial barrier function. Exp Ther Med 21:303 (2021). PubMed: 33717246