Anti-Von Willebrand Factor antibody (ab6994)
Rabbit polyclonal Von Willebrand Factor antibody. Validated in WB, IHC, Flow Cyt, ICC/IF and tested in Rat, Sheep, Horse, Guinea pig, Cow, Dog, Human, Pig. Cited in 195 publication(s).
- Datasheet
- References (261)
- Protocols
Overview
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Product name
Anti-Von Willebrand Factor antibody
See all Von Willebrand Factor primary antibodies -
Description
Rabbit polyclonal to Von Willebrand Factor -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-FrFl, IHC-P, IHC-Fr, WB, Flow Cyt, IHC-FoFrmore details -
Species reactivity
Reacts with: Rat, Sheep, Horse, Guinea pig, Cow, Dog, Human, Pig
Does not react with: Chicken -
Immunogen
Full length native protein (purified) corresponding to Human Von Willebrand Factor. Purified from plasma.
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Positive control
- IHC-P: Pig skin tissue; BCG infected guinea pig lung tissue. ICC/IF: Equine exuberant granulation cells WB: Rat liver tissue lysate
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General notes
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other rabbit serum proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab6994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/400. | |
| IHC-FrFl | 1/200. (see Abreview) | |
| IHC-P | 1/200 - 1/400. for IF and 1/1000-1/2000 for ABC methods with HRP conjugates. Perform enzymatic antigen retrieval with 0.1% pronase for 10 min at 35 °C before commencing with IHC protocol. Indirect Immunofluorescence: minimum working dilution of 1:200 was determined using FFPE sections of human tongue with FITC-conjugated secondary. Indirect Immunoperoxidase Labeling: minimum working dilution of 1:800 was determined using FFPE sections of human tongue with biotinylated secondary and signal amplification. | |
| IHC-Fr | 1/1000. | |
| WB | 1/500. | |
| Flow Cyt | Use a concentration of 5 µg/ml. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-FoFr | Use at an assay dependent concentration. PubMed: 19622235 |
Target
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Function
Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma. -
Tissue specificity
Plasma. -
Involvement in disease
Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII. -
Sequence similarities
Contains 1 CTCK (C-terminal cystine knot-like) domain.
Contains 4 TIL (trypsin inhibitory-like) domains.
Contains 3 VWFA domains.
Contains 3 VWFC domains.
Contains 4 VWFD domains. -
Domain
The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules. -
Post-translational
modificationsAll cysteine residues are involved in intrachain or interchain disulfide bonds.
N- and O-glycosylated. -
Cellular localization
Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules. - Information by UniProt
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Database links
- Entrez Gene: 7450 Human
- Entrez Gene: 116669 Rat
- Omim: 613160 Human
- SwissProt: P04275 Human
- SwissProt: Q62935 Rat
- Unigene: 440848 Human
- Unigene: 35561 Rat
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Alternative names
- Coagulation factor VIII antibody
- Coagulation factor VIII VWF antibody
- F8VWF antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Von Willebrand Factor antibody (ab6994)Immunocytochemical analysis labeling Von Willebrand Factor in equine exuberant granulation cells with ab6994 at 1/100 dilution.
The nuclear counterstain is DAPI(blue)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (ab6994)This image is courtesy of an anonymous Abreviewab6994 staining Von Willebrand Factor in pig skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was enzymatic using pronase, 1mg/ml. Samples were incubated with primary antibody (1/500 in PBS) for 12 hours at 4°C. A Biotin-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
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Western blot - Anti-Von Willebrand Factor antibody (ab6994)This image is courtesy of an anonymous Abreview.All lanes : Anti-Von Willebrand Factor antibody (ab6994) at 1/500 dilution
All lanes : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit-HRP polyclonal at 1/3000 dilution
Performed under reducing conditions.
Observed band size: 300 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesBlocking buffer: 5% milk
Diluting buffer 5% Milk in PBS T20 -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (ab6994)This picture shows Factor VII Immunohistochemical localization in BCG infected Guinea Pig Lung. The image was kindly supplied as part of the review submitted by Elizabeth Chlipala.
Protocols
References
This product has been referenced in:
- D'Onofrio N et al. MicroRNA-33 and SIRT1 influence the coronary thrombus burden in hyperglycemic STEMI patients. J Cell Physiol 235:1438-1452 (2020). Read more (PubMed: 31294459) »
- Chaskiel L et al. Interleukin-1 reduces food intake and body weight in rat by acting in the arcuate hypothalamus. Brain Behav Immun 81:560-573 (2019). Read more (PubMed: 31310797) »
