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  1. Link

    von-willebrand-factor-antibody-ab6994.pdf

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Cardiovascular Blood Serum Proteins
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Anti-Von Willebrand Factor antibody (ab6994)

  • Datasheet
  • SDS
Reviews (60)Q&A (26)References (385)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (ab6994)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (ab6994)

Key features and details

  • Rabbit polyclonal to Von Willebrand Factor
  • Suitable for: WB, ICC/IF, IHC-Fr, Flow Cyt, IHC-P, IHC-FoFr, IHC-FrFl
  • Reacts with: Rat, Sheep, Horse, Guinea pig, Cow, Dog, Human, Pig
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Von Willebrand Factor antibody [EPSISR15] (ab154193)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Von Willebrand Factor antibody
    See all Von Willebrand Factor primary antibodies
  • Description

    Rabbit polyclonal to Von Willebrand Factor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Fr, Flow Cyt, IHC-P, IHC-FoFr, IHC-FrFlmore details
  • Species reactivity

    Reacts with: Rat, Sheep, Horse, Guinea pig, Cow, Dog, Human, Pig
    Does not react with: Chicken
  • Immunogen

    Full length native protein (purified) corresponding to Human Von Willebrand Factor. Purified from plasma.

  • Positive control

    • IHC-P: Human kidney tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    Whole antiserum is fractionated and then further purified by ion exchange chromatography to provide the IgG fraction of antiserum. This fraction is essentially free of other rabbit serum proteins.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Cardiovascular
    • Blood
    • Platelets
    • Cardiovascular
    • Blood
    • Coagulation
    • Extrinsic
    • Cardiovascular
    • Blood
    • Coagulation
    • Regulatory
    • Cancer
    • Invasion/microenvironment
    • Angiogenesis
    • Angiogenic growth factors
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Von Willebrand Factor protein (Tagged) (ab152801)
  • Related Products

    • Anti-CD31 antibody [P2B1] (ab24590)
    • Anti-CD45 antibody [F10-89-4] - Hematopoietic Stem Cell Marker (ab30470)
    • Anti-CD34 antibody [MEC 14.7] (ab8158)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (10)
Use at an assay dependent concentration.
ICC/IF (8)
Use at an assay dependent concentration.
IHC-Fr (14)
Use at an assay dependent concentration.
Flow Cyt (1)
Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P (22)
1/200 - 1/400. for IF and 1/1000-1/2000 for ABC methods with HRP conjugates. Perform enzymatic antigen retrieval with 0.1% pronase for 10 min at 35 °C before commencing with IHC protocol. Indirect Immunofluorescence: minimum working dilution of 1:200 was determined using FFPE sections of human tongue with FITC-conjugated secondary. Indirect Immunoperoxidase Labeling: minimum working dilution of 1:800 was determined using FFPE sections of human tongue with biotinylated secondary and signal amplification.
IHC-FoFr (3)
Use at an assay dependent concentration. PubMed: 19622235
IHC-FrFl (2)
Use at an assay dependent concentration.

(see Abreview)

Notes
WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/200 - 1/400. for IF and 1/1000-1/2000 for ABC methods with HRP conjugates. Perform enzymatic antigen retrieval with 0.1% pronase for 10 min at 35 °C before commencing with IHC protocol. Indirect Immunofluorescence: minimum working dilution of 1:200 was determined using FFPE sections of human tongue with FITC-conjugated secondary. Indirect Immunoperoxidase Labeling: minimum working dilution of 1:800 was determined using FFPE sections of human tongue with biotinylated secondary and signal amplification.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19622235
IHC-FrFl
Use at an assay dependent concentration.

(see Abreview)

Target

  • Function

    Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
  • Sequence similarities

    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 4 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFA domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • Domain

    The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
  • Post-translational
    modifications

    All cysteine residues are involved in intrachain or interchain disulfide bonds.
    N- and O-glycosylated.
  • Cellular localization

    Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
  • Target information above from: UniProt accession P04275 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7450 Human
    • Entrez Gene: 116669 Rat
    • Omim: 613160 Human
    • SwissProt: P04275 Human
    • SwissProt: Q62935 Rat
    • Unigene: 440848 Human
    • Alternative names

      • Coagulation factor VIII antibody
      • Coagulation factor VIII VWF antibody
      • F8VWF antibody
      • Factor VIII related antigen antibody
      • von Willebrand antigen 2 antibody
      • von Willebrand antigen II antibody
      • Von Willebrand disease antibody
      • Von Willebrand factor precursor antibody
      • VWD antibody
      • vWF antibody
      • VWF_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (ab6994)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (ab6994)

      Immunohistochemical analysis of Formalin fixed paraffin-embedded sections human kidney tissue labeling Von Willebrand Factor with ab6994 at 1/2000. 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (ab6994)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Von Willebrand Factor antibody (ab6994)
      Immunohistochemical analysis of Formalin fixed paraffin-embedded sections human kidney tissue labeling Von Willebrand Factor with ab6994 at 1/2000. 

       

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (385)

    Publishing research using ab6994? Please let us know so that we can cite the reference in this datasheet.

    ab6994 has been referenced in 385 publications.

    • Hørlyck S  et al. ATP induces contraction of cultured brain capillary pericytes via activation of P2Y-type purinergic receptors. Am J Physiol Heart Circ Physiol 320:H699-H712 (2021). PubMed: 33306443
    • Huang S  et al. Co-expression of tissue kallikrein 1 and tissue inhibitor of matrix metalloproteinase 1 improves myocardial ischemia-reperfusion injury by promoting angiogenesis and inhibiting oxidative stress. Mol Med Rep 23:N/A (2021). PubMed: 33355364
    • Rosendorf J  et al. Reinforcement of Colonic Anastomosis with Improved Ultrafine Nanofibrous Patch: Experiment on Pig. Biomedicines 9:N/A (2021). PubMed: 33494257
    • Shiroma ME  et al. Pretreatment with melatonin improves ovarian tissue cryopreservation for transplantation. Reprod Biol Endocrinol 19:17 (2021). PubMed: 33536029
    • Liu MM  et al. Diammonium glycyrrhizinate lipid ligand ameliorates lipopolysaccharide-induced acute lung injury by modulating vascular endothelial barrier function. Exp Ther Med 21:303 (2021). PubMed: 33717246
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

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    1-10 of 60 Abreviews

    Immunohistochemistry (Frozen sections) abreview for Anti-Von Willebrand Factor antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (optic nerve)
    Permeabilization
    Yes - 0.25% tritonX100
    Specification
    optic nerve
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Apr 30 2021

    Immunohistochemistry (Frozen sections) abreview for Anti-Von Willebrand Factor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Connective tissue)
    Specification
    Connective tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    DR. Tina Yuan

    Verified customer

    Submitted Jan 05 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Von Willebrand Factor antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Frontal Cortex (Brain))
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization
    No
    Specification
    Frontal Cortex (Brain)
    Blocking step
    Donkey Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 10 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Von Willebrand Factor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Endothelial)
    Permeabilization
    Yes - Triton-X 100
    Specification
    Endothelial
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Formalin
    Read More

    MR. Bryan James

    Verified customer

    Submitted Jun 17 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Von Willebrand Factor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Human Brain Cortex)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9
    Specification
    Human Brain Cortex
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 05 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Von Willebrand Factor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (Kidney)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Protease 2 (Ventana)
    Permeabilization
    No
    Specification
    Kidney
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 31 2017

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Von Willebrand Factor antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Antigen retrieval step
    None
    Permeabilization
    Yes - Triton-X100
    Specification
    Spinal cord
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. K. A.

    Verified customer

    Submitted Sep 30 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Von Willebrand Factor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (kidney)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Miss. Jin Young Park

    Verified customer

    Submitted Aug 26 2016

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Von Willebrand Factor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.3% triton-x-100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 15 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Von Willebrand Factor antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Cat Tissue sections (Cerebellum)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Proteinase K
    Permeabilization
    No
    Specification
    Cerebellum
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More

    Dr. K. A.

    Verified customer

    Submitted Aug 21 2015

    1-10 of 60 Abreviews

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