Overview

  • Product name
    Anti-Von Willebrand Factor antibody
    See all Von Willebrand Factor primary antibodies
  • Description
    Rabbit polyclonal to Von Willebrand Factor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Factor VIII related antigen isolated from human plasma.

Properties

Applications

Our Abpromise guarantee covers the use of ab9378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/50 - 1/100.
Flow Cyt 1/250.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa (predicted molecular weight: 309 kDa).

Target

  • Function
    Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
  • Tissue specificity
    Plasma.
  • Involvement in disease
    Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
  • Sequence similarities
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 4 TIL (trypsin inhibitory-like) domains.
    Contains 3 VWFA domains.
    Contains 3 VWFC domains.
    Contains 4 VWFD domains.
  • Domain
    The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
  • Post-translational
    modifications
    All cysteine residues are involved in intrachain or interchain disulfide bonds.
    N- and O-glycosylated.
  • Cellular localization
    Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • Coagulation factor VIII antibody
    • Coagulation factor VIII VWF antibody
    • F8VWF antibody
    • Factor VIII related antigen antibody
    • von Willebrand antigen 2 antibody
    • von Willebrand antigen II antibody
    • Von Willebrand disease antibody
    • VWD antibody
    • vWF antibody
    • VWF_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling Von Willebrand Factor with ab9378.

  • Anti-Von Willebrand Factor antibody (ab9378) at 1 µg/ml + Lung (Human) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 309 kDa
    Observed band size: 250 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 40 kDa, 50 kDa, 65 kDa. We are unsure as to the identity of these extra bands.



    Many bands of varying sizes can be seen on Western blots, perhaps indicating differential processing by ADAMTS13 and other enzymes
  • Central Panel: ab9378 staining Von Willebrand Factor in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 + 2% BSA in PBS and blocked with 2% BSA for 1 hour at 22°C. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37°C. An Alexa Fluor® 568-conjugated Goat polyclonal to rabbit IgG, dilution 1/100, was used as secondary antibody.
    Top panel: Nuclei counterstained with DAPI (blue).
    Bottom panel: Overlay

    See Abreview

  • ab9378 staining Von Willebrand Factor in Human platelet cells by Flow cytometry.
    Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated chicken anti-rabbit IgG (H+L) at a 1/500 dilution.

    P : Permeabilized;
    US : Unstained, Red Peak;
    IGG RB : IgG Rabbit (Isotype Control), Blue Peak;
    VWF : Von Willebrand Factor antibody, Green peak.

    See Abreview

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining Von Willebrand Factor with ab9378.

References

This product has been referenced in:
  • Smeda M  et al. Dual antiplatelet therapy with clopidogrel and aspirin increases mortality in 4T1 metastatic breast cancer-bearing mice by inducing vascular mimicry in primary tumour. Oncotarget 9:17810-17824 (2018). Read more (PubMed: 29707148) »
  • Schmidt K  et al. The H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice. Elife 7:N/A (2018). Read more (PubMed: 29916805) »
See all 12 Publications for this product

Customer reviews and Q&As

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1-8 of 8 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lung of adult mice)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
No
Specification
Lung of adult mice
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jan 10 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mammary gland, blood vessel)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citrate
Permeabilization
Yes - 0.001% Tween-20
Specification
mammary gland, blood vessel
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Formaldehyde

Lucas Tomko

Verified customer

Submitted Nov 02 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (frozen bone)
Antigen retrieval step
None
Permeabilization
Yes - permeabilization
Specification
frozen bone
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 14 2015

Application
Western blot
Sample
Human Tissue lysate - whole (liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Jul 13 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Rat Tissue sections (sciatic nerve)
Specification
sciatic nerve
Permeabilization
Yes - 0.3% TritonX-100
Fixative
Paraformaldehyde

Hye Yeong Lee

Verified customer

Submitted Mar 16 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Platelets)
Loading amount
20 µg
Specification
Platelets
Treatment
ADP for 30 min
Gel Running Conditions
Reduced Denaturing (8-16% Biorad Criterion Gel with 18 wells)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (Platelets)
Specification
Platelets
Preparation
Cell harvesting/tissue preparation method: PL were isolated spinning Platelet rich plasma on Histopaque
Sample buffer: PBS
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton-X100 in 2% BSA for 15min
Gating Strategy
Platelets

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (PMN)
Specification
PMN
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TritonX-100 + 2% BSA in PBS for 15 min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C

Dr. Mahesh Shivananjappa

Verified customer

Submitted Dec 22 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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