Recombinant
RabMAb

Recombinant Anti-Von Willebrand Factor antibody [EPR12010] (ab179451)

Overview

  • Product name

    Anti-Von Willebrand Factor antibody [EPR12010]
    See all Von Willebrand Factor primary antibodies
  • Description

    Rabbit monoclonal [EPR12010] to Von Willebrand Factor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Von Willebrand Factor aa 1200-1300. The exact sequence is proprietary.
    Database link: P04275

  • Positive control

    • Human fetal liver, serum and plasma lysates; human brain and tonsil tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 309 kDa.
IHC-P 1/250 - 1/500.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
    • Tissue specificity

      Plasma.
    • Involvement in disease

      Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.
    • Sequence similarities

      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 4 TIL (trypsin inhibitory-like) domains.
      Contains 3 VWFA domains.
      Contains 3 VWFC domains.
      Contains 4 VWFD domains.
    • Domain

      The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.
    • Post-translational
      modifications

      All cysteine residues are involved in intrachain or interchain disulfide bonds.
      N- and O-glycosylated.
    • Cellular localization

      Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
    • Information by UniProt
    • Database links

    • Alternative names

      • Coagulation factor VIII antibody
      • Coagulation factor VIII VWF antibody
      • F8VWF antibody
      • Factor VIII related antigen antibody
      • von Willebrand antigen 2 antibody
      • von Willebrand antigen II antibody
      • Von Willebrand disease antibody
      • Von Willebrand factor precursor antibody
      • VWD antibody
      • vWF antibody
      • VWF_HUMAN antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Von Willebrand Factor with ab179451 at 1/250 dilution.

    • All lanes : Anti-Von Willebrand Factor antibody [EPR12010] (ab179451) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human serum lysate
      Lane 3 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 309 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Von Willebrand Factor with ab179451 at 1/250 dilution.

    References

    ab179451 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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