RecombinantRabMAb

Recombinant Anti-Von Willebrand Factor antibody [EPR12011] (ab174290)

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Western blot - Anti-Von Willebrand Factor antibody [EPR12011] (ab174290)
  • Western blot - Anti-Von Willebrand Factor antibody [EPR12011] (ab174290)
  • Anti-Von Willebrand Factor antibody [EPR12011] (ab174290)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12011] to Von Willebrand Factor
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Von Willebrand Factor antibody [EPR12011]
    See all Von Willebrand Factor primary antibodies

  • Description

    Rabbit monoclonal [EPR12011] to Von Willebrand Factor

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Von Willebrand Factor aa 2050-2150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P04275

  • Positive control

    • WB: Human serum, Human plasma, Rat plasma, and Mouse platelet lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab174290 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 309 kDa.
  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.

    • Target

    • Function

      Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex GPIb-IX-V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.

    • Tissue specificity

      Plasma.

    • Involvement in disease

      Defects in VWF are the cause of von Willebrand disease (VWD) [MIM:277480]. VWD defines a group of hemorrhagic disorders in which the von Willebrand factor is either quantitatively or qualitatively abnormal resulting in altered platelet function. Symptoms vary depending on severity and disease type but may include prolonged bleeding time, deficiency of factor VIII and impaired platelet adhesion. Type I von Willebrand disease is the most common form and is characterized by partial quantitative plasmatic deficiency of an otherwise structurally and functionally normal Willebrand factor; type II is associated with a qualitative deficiency and functional anomalies of the Willebrand factor; type III is the most severe form and is characterized by total or near-total absence of Willebrand factor in the plasma and cellular compartments, also leading to a profound deficiency of plasmatic factor VIII.

    • Sequence similarities

      Contains 1 CTCK (C-terminal cystine knot-like) domain.
      Contains 4 TIL (trypsin inhibitory-like) domains.
      Contains 3 VWFA domains.
      Contains 3 VWFC domains.
      Contains 4 VWFD domains.

    • Domain

      The von Willebrand antigen 2 is required for multimerization of vWF and for its targeting to storage granules.

    • Post-translational
      modifications

      All cysteine residues are involved in intrachain or interchain disulfide bonds.
      N- and O-glycosylated.

    • Cellular localization

      Secreted. Secreted > extracellular space > extracellular matrix. Localized to storage granules.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Coagulation factor VIII antibody
      • Coagulation factor VIII VWF antibody
      • F8VWF antibody
      • Factor VIII related antigen antibody
      • von Willebrand antigen 2 antibody
      • von Willebrand antigen II antibody
      • Von Willebrand disease antibody
      • Von Willebrand factor precursor antibody
      • VWD antibody
      • vWF antibody
      • VWF_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Von Willebrand Factor antibody [EPR12011] (ab174290)
      Western blot - Anti-Von Willebrand Factor antibody [EPR12011] (ab174290)
      All lanes : Anti-Von Willebrand Factor antibody [EPR12011] (ab174290) at 1/1000 dilution (Purified)

      Lane 1 : Human serum lysates
      Lane 2 : Human plasma lysates
      Lane 3 : Rat plasma lysates
      Lane 4 : Mouse platelet lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 309 kDa
      Observed band size: 309 kDa



      We are unsure how to define the extra bands.

    • Western blot - Anti-Von Willebrand Factor antibody [EPR12011] (ab174290)
      Western blot - Anti-Von Willebrand Factor antibody [EPR12011] (ab174290)
      All lanes : Anti-Von Willebrand Factor antibody [EPR12011] (ab174290) at 1/1000 dilution

      Lane 1 : Human serum lysate
      Lane 2 : Human plasma lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 309 kDa

    • Anti-Von Willebrand Factor antibody [EPR12011] (ab174290)
      Anti-Von Willebrand Factor antibody [EPR12011] (ab174290)

    References (1)

    Publishing research using ab174290? Please let us know so that we can cite the reference in this datasheet.

    ab174290 has been referenced in 1 publication.

    • Luo X  et al. Caveolin 1-related autophagy initiated by aldosterone-induced oxidation promotes liver sinusoidal endothelial cells defenestration. Redox Biol 13:508-521 (2017). PubMed: 28734243

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