Product nameAnti-VP16 tag antibody
DescriptionRabbit polyclonal to VP16 tag
SpecificityThis antibody was raised against an immunogen that is predicted to react with HSV-1. We do not expect this product to react with HSV-2. Abcam welcomes customer feedback and would appreciate any comments regarding this product.
Tested applicationsSuitable for: WB, IHC-P, IHC-Frmore details
Species reactivityReacts with: Species independent
Synthetic peptide derived from within the activation domain (amino acids 413-490) of HSV VP16.
- This antibody recognises GAL4-VP16 recombinant fusion protein (30kDa) in Western blot.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab4808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Detects a band of approximately 30 kDa.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
ab4808 at a 1/100 dilution staining mouse brain sections by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). Following heat mediated antigen retrieval, the antibody was incubated with the tissue for 24 hours. Bound antibody was detected using an Alexa-Fluor 488 ® conjugated Donkey anti-Rabbit IgG.
This image is courtesy of an Abreview submitted on 10 March 2006.
Lane 1 : Marker
Lanes 2-3 : Anti-VP16 tag antibody (ab4808) at 1 µg/ml
Lane 2 : GAL4-VP16 human recombinant E.coli
Lane 3 : GAL4-VP16 human recombinant E.coli
with VP16 tag peptide (ab13406) at 1 µg/ml
Lanes 2-3 : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution
Performed under reducing conditions.
This product has been referenced in:
- Wang L et al. Two Paralogous Tetraspanins TSP-12 and TSP-14 Function with the ADAM10 Metalloprotease SUP-17 to Promote BMP Signaling in Caenorhabditis elegans. PLoS Genet 13:e1006568 (2017). Read more (PubMed: 28068334) »
- Fehrenbacher N et al. The G protein-coupled receptor GPR31 promotes membrane association of KRAS. J Cell Biol 216:2329-2338 (2017). Read more (PubMed: 28619714) »