Overview

  • Product name

    Anti-VPAC1 antibody [SP234]
    See all VPAC1 primary antibodies
  • Description

    Rabbit monoclonal [SP234] to VPAC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Pig
  • Immunogen

    Synthetic peptide within Human VPAC1 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: P32241

  • Positive control

    • IHC-P: Human tonsil, and Rat spleen tissue; FC: A431 cells.
  • General notes

     This product was previously labelled as VIP Receptor 1, VIPR1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab183312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/100.

Primary antibody incubation for 30 minutes at 4°C.

IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

Target

  • Function

    This is a receptor for VIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The affinity is VIP = PACAP-27 > PACAP-38.
  • Tissue specificity

    In lung, HT29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and Tsup-1, but not in the T-cell lines PEER, MOLT-4, HSB and YT.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ41949 antibody
    • HVR 1 antibody
    • HVR1 antibody
    • PACAP R 2 antibody
    • PACAP type II receptor antibody
    • PACAP-R-2 antibody
    • PACAP-R2 antibody
    • Pituitary adenylate cyclase activating polypeptide receptor type II antibody
    • Pituitary adenylate cyclase activating polypeptide type II receptor antibody
    • Pituitary adenylate cyclase-activating polypeptide type II receptor antibody
    • RCD1 antibody
    • RDC 1 antibody
    • RDC1 antibody
    • V1RG antibody
    • VAPC 1 antibody
    • VAPC1 antibody
    • Vasoactive intestinal peptide receptor 1 antibody
    • Vasoactive intestinal polypeptide receptor 1 antibody
    • Vasoactive intestinal polypeptide receptor 1 precursor antibody
    • VIP and PACAP receptor 1 antibody
    • VIP R 1 antibody
    • VIP receptor type I antibody
    • VIP-R-1 antibody
    • VIPR 1 antibody
    • VIPR antibody
    • VIPR1 antibody
    • VIPR1_HUMAN antibody
    • VIRG antibody
    • VPAC 1 antibody
    • VPAC1 antibody
    • VPCAP1R antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling VPAC1 with ab183312 at 1/100 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling VPAC1 with ab183312 at 1/100 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Formalin-fixed, paraffin-embedded human tonsil tissue stained for VPAC1 using ab183312 at 1/100 dilution in immunohistochemical analysis.

  • Flow cytometric analysis of A431 (Human epidermoid carcinoma cell line) cells labeling VPAC1 using ab183312 at 1/100 dilution (green) compared to a negative control rabbit IgG (blue)

References

ab183312 has not yet been referenced specifically in any publications.

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