Recombinant
RabMAb

Recombinant Anti-VPAC1 antibody [SP234] - BSA and Azide free (ab245743)

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Overview

  • Product name

    Anti-VPAC1 antibody [SP234] - BSA and Azide free
    See all VPAC1 primary antibodies

  • Description

    Rabbit monoclonal [SP234] to VPAC1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig

  • Immunogen

    Synthetic peptide within Human VPAC1 aa 400 to the C-terminus. The exact sequence is proprietary.
    Database link: P32241

  • Positive control

    • IHC-P: Human tonsil, and Rat spleen tissue; FC: A431 cells.

  • General notes

    ab245743 is the carrier-free version of ab183312 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab245743 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as VIP Receptor 1, VIPR1

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab245743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

 
Flow Cyt Use at an assay dependent concentration.

Primary antibody incubation for 30 minutes at 4 degrees.

 

Target

  • Function

    This is a receptor for VIP. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The affinity is VIP = PACAP-27 > PACAP-38.

  • Tissue specificity

    In lung, HT29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and Tsup-1, but not in the T-cell lines PEER, MOLT-4, HSB and YT.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • FLJ41949 antibody
    • HVR 1 antibody
    • HVR1 antibody
    • PACAP R 2 antibody
    • PACAP type II receptor antibody
    • PACAP-R-2 antibody
    • PACAP-R2 antibody
    • Pituitary adenylate cyclase activating polypeptide receptor type II antibody
    • Pituitary adenylate cyclase activating polypeptide type II receptor antibody
    • Pituitary adenylate cyclase-activating polypeptide type II receptor antibody
    • RCD1 antibody
    • RDC 1 antibody
    • RDC1 antibody
    • V1RG antibody
    • VAPC 1 antibody
    • VAPC1 antibody
    • Vasoactive intestinal peptide receptor 1 antibody
    • Vasoactive intestinal polypeptide receptor 1 antibody
    • Vasoactive intestinal polypeptide receptor 1 precursor antibody
    • VIP and PACAP receptor 1 antibody
    • VIP R 1 antibody
    • VIP receptor type I antibody
    • VIP-R-1 antibody
    • VIPR 1 antibody
    • VIPR antibody
    • VIPR1 antibody
    • VIPR1_HUMAN antibody
    • VIRG antibody
    • VPAC 1 antibody
    • VPAC1 antibody
    • VPCAP1R antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] - BSA and Azide free (ab245743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] - BSA and Azide free (ab245743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling VPAC1 with ab183312 at 1/100 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183312)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] - BSA and Azide free (ab245743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] - BSA and Azide free (ab245743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling VPAC1 with ab183312 at 1/100 dilution (1.01 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183312)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] - BSA and Azide free (ab245743)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC1 antibody [SP234] - BSA and Azide free (ab245743)

    Formalin-fixed, paraffin-embedded human tonsil tissue stained for VPAC1 using ab183312 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183312)

  • Flow Cytometry - Anti-VPAC1 antibody [SP234] - BSA and Azide free (ab245743)
    Flow Cytometry - Anti-VPAC1 antibody [SP234] - BSA and Azide free (ab245743)

    Flow cytometric analysis of A431 (Human epidermoid carcinoma cell line) cells labeling VPAC1 using ab183312 at 1/100 dilution (green) compared to a negative control rabbit IgG (blue)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab183312)

References

This product has been referenced in:

  • Li S  et al. VIP activates primordial follicles of rat through ERK-mTOR pathway during in tissue culture. Reproduction N/A:N/A (2019). Read more (PubMed: 30817320) »
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