Key features and details
- Rabbit polyclonal to VPAC2
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab28624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
FunctionThis is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C.
Tissue specificityExpressed in CD4+ T-cells, but not in CD8+ T-cells. Expressed in the T-cell lines Jurkat, PEER, MOLT-4, HSB, YT and Tsup-1, but not in the T-cell lines HARRIS and HUT 78.
Sequence similaritiesBelongs to the G-protein coupled receptor 2 family.
Cellular localizationCell membrane.
- Information by UniProt
- Helodermin-preferring VIP receptor antibody
- PACAP type III receptor antibody
- PACAP-R-3 antibody
ab28624 staining Human VIP Receptor 2 in human neuroendocrine cells of the small intestine by immunohistochemistry using paraffin embedded tissue.
ICC/IF image of ab28624 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28624, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab28624 has been referenced in 9 publications.
- Ivic I et al. VPAC1 receptors play a dominant role in PACAP-induced vasorelaxation in female mice. PLoS One 14:e0211433 (2019). PubMed: 30682157
- Jones JR et al. SCN VIP Neurons Are Essential for Normal Light-Mediated Resetting of the Circadian System. J Neurosci 38:7986-7995 (2018). PubMed: 30082421
- Liu D et al. mTOR signaling in VIP neurons regulates circadian clock synchrony and olfaction. Proc Natl Acad Sci U S A 115:E3296-E3304 (2018). PubMed: 29555746
- Bertolini M et al. Vasoactive intestinal peptide, whose receptor-mediated signalling may be defective in alopecia areata, provides protection from hair follicle immune privilege collapse. Br J Dermatol 175:531-41 (2016). PubMed: 27059672
- Doi M et al. Gpr176 is a Gz-linked orphan G-protein-coupled receptor that sets the pace of circadian behaviour. Nat Commun 7:10583 (2016). PubMed: 26882873
- Juhász T et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci 16:17344-67 (2015). PubMed: 26230691
- Miller JE et al. Vasoactive intestinal polypeptide mediates circadian rhythms in mammalian olfactory bulb and olfaction. J Neurosci 34:6040-6 (2014). PubMed: 24760863
- An S et al. Spatiotemporal distribution of vasoactive intestinal polypeptide receptor 2 in mouse suprachiasmatic nucleus. J Comp Neurol 520:2730-41 (2012). PubMed: 22684939
- Ortner A et al. VPAC receptor mediated tumor cell targeting by protamine based nanoparticles. J Drug Target 18:457-67 (2010). ICC ; Human . PubMed: 20050817