• Product name
  • Description
    Rabbit polyclonal to VPAC2
  • Host species
  • Tested applications
    Suitable for: ICC/IF, ICC, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:


    conjugated to KLH, corresponding to amino acids 419-438 of Human VIP Receptor 2

  • Positive control
    • Membrane preparations for HEK 293 cells stably expressing VPAC2.



Our Abpromise guarantee covers the use of ab28624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
ICC 1/1000.
IHC-P 1/500.
WB 1/1000. Detects a band of approximately 50-60 kDa.


  • Function
    This is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C.
  • Tissue specificity
    Expressed in CD4+ T-cells, but not in CD8+ T-cells. Expressed in the T-cell lines Jurkat, PEER, MOLT-4, HSB, YT and Tsup-1, but not in the T-cell lines HARRIS and HUT 78.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Helodermin-preferring VIP receptor antibody
    • PACAP type III receptor antibody
    • PACAP-R-3 antibody
    • PACAP-R3 antibody
    • Pituitary adenylate cyclase-activating polypeptide type III receptor antibody
    • Vasoactive intestinal polypeptide receptor 2 antibody
    • VIP Receptor 2 antibody
    • VIP-R-2 antibody
    • Vipr2 antibody
    • VIPR2_HUMAN antibody
    • VPAC2 receptor antibody
    see all


  • ab28624 staining Human VIP Receptor 2 in human neuroendocrine cells of the small intestine by immunohistochemistry using paraffin embedded tissue.

  • ICC/IF image of ab28624 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28624, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Ivic I  et al. VPAC1 receptors play a dominant role in PACAP-induced vasorelaxation in female mice. PLoS One 14:e0211433 (2019). Read more (PubMed: 30682157) »
  • Liu D  et al. mTOR signaling in VIP neurons regulates circadian clock synchrony and olfaction. Proc Natl Acad Sci U S A 115:E3296-E3304 (2018). Read more (PubMed: 29555746) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 2% · Temperature: 25°C
Rat Cell (Suprachiamatic Nucleus)
Suprachiamatic Nucleus
Yes - Triton-X

Abcam user community

Verified customer

Submitted Aug 27 2013


Thank you for your inquiry.

I am double-checking with the lab about ab16155, but I can tell you that for ab28624 the protocol used citrate buffer:

Briefly, sections were dewaxed, microwaved in 10 mmol/L citric acid (pH 6.0) for 20 minutes at 600 W, and subsequently incubated with 2 ug/mLovernight at 4°C. Staining of primary antibody was detected with biotinylated goat anti-rabbit IgG followed by an incubation with avidin-biotinylated peroxidase solution. Tissue was then rinsed and stained with 3,3-diaminobenzidineglucose oxidase for 15 minutes.

Once I have more information about ab16155, I will let you know.

I hope this information helps.

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