Anti-VPAC2 antibody (ab28624)
Key features and details
- Rabbit polyclonal to VPAC2
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-VPAC2 antibody
See all VPAC2 primary antibodies -
Description
Rabbit polyclonal to VPAC2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
LQFHRGSRAQSFLQTETSVI
conjugated to KLH, corresponding to amino acids 419-438 of Human VIP Receptor 2 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/500.
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WB |
1/250 - 1/500. Predicted molecular weight: 49 kDa.
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Notes |
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IHC-P
1/500. |
WB
1/250 - 1/500. Predicted molecular weight: 49 kDa. |
Target
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Function
This is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C. -
Tissue specificity
Expressed in CD4+ T-cells, but not in CD8+ T-cells. Expressed in the T-cell lines Jurkat, PEER, MOLT-4, HSB, YT and Tsup-1, but not in the T-cell lines HARRIS and HUT 78. -
Sequence similarities
Belongs to the G-protein coupled receptor 2 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 7434 Human
- Omim: 601970 Human
- SwissProt: P41587 Human
- Unigene: 585052 Human
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Alternative names
- Helodermin-preferring VIP receptor antibody
- PACAP type III receptor antibody
- PACAP-R-3 antibody
see all
Images
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Anti-VPAC2 antibody (ab28624) at 1/500 dilution + PC-3 cell lysate at 30 µg
Secondary
Goat anti-Rabbit IgG (H+L), HRP conjugate at 1/4000 dilution
Predicted band size: 49 kDa -
ab28624 staining Human VIP Receptor 2 in human neuroendocrine cells of the small intestine by immunohistochemistry using paraffin embedded tissue.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab28624 has been referenced in 9 publications.
- Ivic I et al. VPAC1 receptors play a dominant role in PACAP-induced vasorelaxation in female mice. PLoS One 14:e0211433 (2019). PubMed: 30682157
- Jones JR et al. SCN VIP Neurons Are Essential for Normal Light-Mediated Resetting of the Circadian System. J Neurosci 38:7986-7995 (2018). PubMed: 30082421
- Liu D et al. mTOR signaling in VIP neurons regulates circadian clock synchrony and olfaction. Proc Natl Acad Sci U S A 115:E3296-E3304 (2018). PubMed: 29555746
- Bertolini M et al. Vasoactive intestinal peptide, whose receptor-mediated signalling may be defective in alopecia areata, provides protection from hair follicle immune privilege collapse. Br J Dermatol 175:531-41 (2016). PubMed: 27059672
- Doi M et al. Gpr176 is a Gz-linked orphan G-protein-coupled receptor that sets the pace of circadian behaviour. Nat Commun 7:10583 (2016). PubMed: 26882873
- Juhász T et al. Pituitary Adenylate Cyclase Activating Polypeptide (PACAP) Pathway Is Induced by Mechanical Load and Reduces the Activity of Hedgehog Signaling in Chondrogenic Micromass Cell Cultures. Int J Mol Sci 16:17344-67 (2015). PubMed: 26230691
- Miller JE et al. Vasoactive intestinal polypeptide mediates circadian rhythms in mammalian olfactory bulb and olfaction. J Neurosci 34:6040-6 (2014). PubMed: 24760863
- An S et al. Spatiotemporal distribution of vasoactive intestinal polypeptide receptor 2 in mouse suprachiasmatic nucleus. J Comp Neurol 520:2730-41 (2012). PubMed: 22684939
- Ortner A et al. VPAC receptor mediated tumor cell targeting by protamine based nanoparticles. J Drug Target 18:457-67 (2010). ICC ; Human . PubMed: 20050817