Overview

  • Product name
  • Description
    Rabbit polyclonal to VPAC2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, ICC, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    LQFHRGSRAQSFLQTETSVI

    conjugated to KLH, corresponding to amino acids 419-438 of Human VIP Receptor 2

  • Positive control
    • Membrane preparations for HEK 293 cells stably expressing VPAC2.

Properties

Applications

Our Abpromise guarantee covers the use of ab28624 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
ICC 1/1000.
IHC-P 1/500.
WB 1/1000. Detects a band of approximately 50-60 kDa.

Target

  • Function
    This is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C.
  • Tissue specificity
    Expressed in CD4+ T-cells, but not in CD8+ T-cells. Expressed in the T-cell lines Jurkat, PEER, MOLT-4, HSB, YT and Tsup-1, but not in the T-cell lines HARRIS and HUT 78.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 2 family.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Helodermin-preferring VIP receptor antibody
    • PACAP type III receptor antibody
    • PACAP-R-3 antibody
    • PACAP-R3 antibody
    • Pituitary adenylate cyclase-activating polypeptide type III receptor antibody
    • Vasoactive intestinal polypeptide receptor 2 antibody
    • VIP Receptor 2 antibody
    • VIP-R-2 antibody
    • Vipr2 antibody
    • VIPR2_HUMAN antibody
    • VPAC2 receptor antibody
    see all

Images

  • ab28624 staining Human VIP Receptor 2 in human neuroendocrine cells of the small intestine by immunohistochemistry using paraffin embedded tissue.

  • ICC/IF image of ab28624 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28624, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Ivic I  et al. VPAC1 receptors play a dominant role in PACAP-induced vasorelaxation in female mice. PLoS One 14:e0211433 (2019). Read more (PubMed: 30682157) »
  • Liu D  et al. mTOR signaling in VIP neurons regulates circadian clock synchrony and olfaction. Proc Natl Acad Sci U S A 115:E3296-E3304 (2018). Read more (PubMed: 29555746) »
See all 7 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 2% · Temperature: 25°C
Sample
Rat Cell (Suprachiamatic Nucleus)
Specification
Suprachiamatic Nucleus
Permeabilization
Yes - Triton-X
Fixative
Formaldehyde

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Verified customer

Submitted Aug 27 2013

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