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    vpac2-antibody-sp235-ab183334.pdf

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RecombinantRabMAb

Recombinant Anti-VPAC2 antibody [SP235] (ab183334)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)
  • Flow Cytometry - Anti-VPAC2 antibody [SP235] (ab183334)
  • Flow Cytometry - Anti-VPAC2 antibody [SP235] (ab183334)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)
  • Anti-VPAC2 antibody [SP235] (ab183334)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP235] to VPAC2
  • Suitable for: Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Protein
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Recombinant Human VPAC2 protein (ab159805)

View more associated products

Overview

  • Product name

    Anti-VPAC2 antibody [SP235]
    See all VPAC2 primary antibodies
  • Description

    Rabbit monoclonal [SP235] to VPAC2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human liver, hepatocellular carcinoma, liver cirrhosis, Human tonsil, Mouse spleen, and Rat spleen tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP235
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • Hormones
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • More GPCR
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Endocrine system
    • Hypothalamic pituitary adrenal axis
    • Cardiovascular
    • Vasculature
    • Vasodilation
    • Other

Associated products

  • Alternative Versions

    • Anti-VPAC2 antibody [SP235] - BSA and Azide free (ab246352)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Recombinant Human VPAC2 protein (ab159805)
  • Recombinant Protein

    • Recombinant Human VPAC2 protein (ab159805)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183334 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/100.
Notes
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
1/100.

Target

  • Function

    This is a receptor for VIP as well as PACAP-38 and -27, the activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Can be coupled to phospholipase C.
  • Tissue specificity

    Expressed in CD4+ T-cells, but not in CD8+ T-cells. Expressed in the T-cell lines Jurkat, PEER, MOLT-4, HSB, YT and Tsup-1, but not in the T-cell lines HARRIS and HUT 78.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 2 family.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P41587 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7434 Human
    • Entrez Gene: 22355 Mouse
    • Entrez Gene: 29555 Rat
    • Omim: 601970 Human
    • SwissProt: P41587 Human
    • SwissProt: P41588 Mouse
    • SwissProt: P35000 Rat
    • Unigene: 585052 Human
    • Unigene: 395245 Mouse
    • Unigene: 10011 Rat
    see all
  • Alternative names

    • Helodermin-preferring VIP receptor antibody
    • PACAP type III receptor antibody
    • PACAP-R-3 antibody
    • PACAP-R3 antibody
    • Pituitary adenylate cyclase-activating polypeptide type III receptor antibody
    • Vasoactive intestinal polypeptide receptor 2 antibody
    • VIP Receptor 2 antibody
    • VIP-R-2 antibody
    • Vipr2 antibody
    • VIPR2_HUMAN antibody
    • VPAC2 receptor antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling VPAC2 with ab183334 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry - Anti-VPAC2 antibody [SP235] (ab183334)
    Flow Cytometry - Anti-VPAC2 antibody [SP235] (ab183334)

    Flow Cytometry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling VPAC2 with purified ab183334 at 1/20 dilution (5 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

  • Flow Cytometry - Anti-VPAC2 antibody [SP235] (ab183334)
    Flow Cytometry - Anti-VPAC2 antibody [SP235] (ab183334)

    Flow cytometric analysis of Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling VPAC2 using ab183334 (green) compare to negative control of rabbit IgG (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VPAC2 antibody [SP235] (ab183334)

    Immunohistochemical analysis of formalin-fixed, paraffin-embedded human tonsil tissue labeling VPAC2 using ab183334 at a 1/100 dilution.

  • Anti-VPAC2 antibody [SP235] (ab183334)
    Anti-VPAC2 antibody [SP235] (ab183334)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (0)

Publishing research using ab183334? Please let us know so that we can cite the reference in this datasheet.

ab183334 has not yet been referenced specifically in any publications.

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