Key features and details
- Mouse monoclonal [10D7A7B2] to VPH1
- Suitable for: WB, ICC/IF
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG2a
Product nameAnti-VPH1 antibody [10D7A7B2]
DescriptionMouse monoclonal [10D7A7B2] to VPH1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Full length native protein (purified). This information is considered to be commercially sensitive.
This antibody clone is manufactured by Abcam.
This IgG2a kappa isotype control monoclonal antibody was raised in mouse against a yeast-specific protein, V-ATPase subunit (the product of VPH1 gene). It is a suitable isotype control for mammalian species.
Isotype controls are used to confirm that the primary antibody binding is specific and not a result of non-specific Fc receptor binding or other protein interactions. The isotype control antibody should match the primary antibody’s host species, isotype, and possible conjugation.
Product was previously marketed under the MitoSciences sub-brand.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by affinity purification.
Light chain typekappa
Our Abpromise guarantee covers the use of ab113683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).|
|ICC/IF||Use a concentration of 20 µg/ml. Heat induced antigen retrieval recommended.|
FunctionSubunit of the integral membrane V0 complex of vacuolar ATPase essential for assembly and catalytic activity. Is present only in vacuolar V-ATPase complexes. Enzymes containing this subunit have a 4-fold higher ratio of proton transport to ATP hydrolysis than complexes containing the Golgi/endosomal isoform and undergo reversible dissociation of V1 and V0 in response to glucose depletion. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
Sequence similaritiesBelongs to the V-ATPase 116 kDa subunit family.
Cellular localizationVacuole membrane.
- Information by UniProt
- V-ATPase 95 kDa subunit antibody
- V-ATPase a 1 subunit antibody
- V-type proton ATPase subunit a antibody
ab113683 has been referenced in 5 publications.
- Hose J et al. The genetic basis of aneuploidy tolerance in wild yeast. Elife 9:N/A (2020). PubMed: 31909711
- Azevedo C et al. Development of a yeast model to study the contribution of vacuolar polyphosphate metabolism to lysine polyphosphorylation. J Biol Chem 295:1439-1451 (2020). PubMed: 31844018
- Cavellini L et al. An ubiquitin-dependent balance between mitofusin turnover and fatty acids desaturation regulates mitochondrial fusion. Nat Commun 8:15832 (2017). WB ; Saccharomyces cerevisiae . PubMed: 28607491
- Dodgson SE et al. The pleiotropic deubiquitinase Ubp3 confers aneuploidy tolerance. Genes Dev 30:2259-2271 (2016). PubMed: 27807036
- Simpkins JA et al. Disruption of a cystine transporter downregulates expression of genes involved in sulfur regulation and cellular respiration. Biol Open 5:689-97 (2016). PubMed: 27142334