Anti-VPRBP antibody (ab245483)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-VPRBP antibody
See all VPRBP primary antibodies -
Description
Rabbit polyclonal to VPRBP -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human VPRBP aa 25-75. The exact sequence is proprietary. (NP_055518.1).
Database link: Q9Y4B6 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245483 was affinity purified using an epitope specific to VPRBP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab245483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 169 kDa. |
Target
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Relevance
VprBP may act as a receptor for CUL4-DDB1 ubiquitin ligase complex. In case of HIV1 infection, this protein is recruited by HIV1 VPR protein which is believed to result in an arrest of the cell cyccle in the G2 phase. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 9730 Human
- Entrez Gene: 321006 Mouse
- SwissProt: Q9Y4B6 Human
- SwissProt: Q80TR8 Mouse
- Unigene: 716623 Human
- Unigene: 440089 Mouse
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Alternative names
- DCAF1 antibody
- DDB1 and CUL4 associated factor 1 antibody
- HIV-1 Vpr-binding protein antibody
see all
Images
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Western blot - Anti-VPRBP antibody (ab245483)All lanes : Anti-VPRBP antibody (ab245483) at 0.04 µg
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 169 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-VPRBP antibody (ab245483)VPRBP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245483 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245483 at 1 µg/ml.
Lane 1: ab245483 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
Datasheets and documents
References
ab245483 has not yet been referenced specifically in any publications.
