Product nameAnti-VPRBP antibody
See all VPRBP primary antibodies
DescriptionRabbit polyclonal to VPRBP
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human VPRBP aa 25-75. The exact sequence is proprietary. (NP_055518.1).
Database link: Q9Y4B6
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245483 was affinity purified using an epitope specific to VPRBP immobilized on solid support.
Our Abpromise guarantee covers the use of ab245483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 169 kDa.|
RelevanceVprBP may act as a receptor for CUL4-DDB1 ubiquitin ligase complex. In case of HIV1 infection, this protein is recruited by HIV1 VPR protein which is believed to result in an arrest of the cell cyccle in the G2 phase.
Cellular localizationCytoplasmic and Nuclear
- DCAF1 antibody
- DDB1 and CUL4 associated factor 1 antibody
- HIV-1 Vpr-binding protein antibody
All lanes : Anti-VPRBP antibody (ab245483) at 0.04 µg
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 169 kDa
Exposure time: 3 minutes
VPRBP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245483 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245483 at 1 µg/ml.
Lane 1: ab245483 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
ab245483 has not yet been referenced specifically in any publications.