Anti-VPRBP antibody (ab245483)
Key features and details
- Rabbit polyclonal to VPRBP
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-VPRBP antibody
See all VPRBP primary antibodies -
Description
Rabbit polyclonal to VPRBP -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human VPRBP aa 25-75. The exact sequence is proprietary. (NP_055518.1).
Database link: Q9Y4B6 -
Positive control
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysates. IP: HeLa whole cell lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab245483 was affinity purified using an epitope specific to VPRBP immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab245483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-5 µg/mg of lysate. | |
| WB | 1/2000 - 1/10000. Predicted molecular weight: 169 kDa. |
Target
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Relevance
VprBP may act as a receptor for CUL4-DDB1 ubiquitin ligase complex. In case of HIV1 infection, this protein is recruited by HIV1 VPR protein which is believed to result in an arrest of the cell cyccle in the G2 phase. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 9730 Human
- Entrez Gene: 321006 Mouse
- SwissProt: Q9Y4B6 Human
- SwissProt: Q80TR8 Mouse
- Unigene: 716623 Human
- Unigene: 440089 Mouse
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Alternative names
- DCAF1 antibody
- DDB1 and CUL4 associated factor 1 antibody
- HIV-1 Vpr-binding protein antibody
see all
Images
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Western blot - Anti-VPRBP antibody (ab245483)All lanes : Anti-VPRBP antibody (ab245483) at 0.04 µg
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 169 kDa
Exposure time: 3 minutes -
Immunoprecipitation - Anti-VPRBP antibody (ab245483)VPRBP was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab245483 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab245483 at 1 µg/ml.
Lane 1: ab245483 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab245483 has not yet been referenced specifically in any publications.
