• Product name

  • Description

    Rabbit polyclonal to VPS11
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 891-941 of Human VPS11 (NP_068375.3).

  • Positive control

    • 293T, Jurkat and HeLa whole cell lysates.


  • Form

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab125083 was affinity purified using an epitope specific to VPS11 immobilized on solid support.
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab125083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 108 kDa.
IP Use at 2-10 µg/mg of lysate.


  • Function

    May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
  • Tissue specificity

    Ubiquitous. Expression was highest in heart and low in lung.
  • Sequence similarities

    Belongs to the VPS11 family.
    Contains 1 clathrin repeat.
    Contains 1 RING-type zinc finger.
  • Cellular localization

    Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle.
  • Information by UniProt
  • Database links

  • Alternative names

    • END1 antibody
    • HGNC:14583 antibody
    • hVPS11 antibody
    • PEP5 antibody
    • PP3476 antibody
    • RING finger protein 108 antibody
    • RNF108 antibody
    • Vacuolar protein sorting 11 (yeast homolog) antibody
    • Vacuolar protein sorting 11 (yeast) antibody
    • Vacuolar protein sorting 11 homolog (S. cerevisiae) antibody
    • Vacuolar protein sorting 11 homolog antibody
    • Vacuolar protein sorting associated protein 11 homolog antibody
    • Vacuolar protein sorting protein 11 antibody
    • Vacuolar protein sorting-associated protein 11 homolog antibody
    • vps11 antibody
    • VPS11_HUMAN antibody
    see all


  • All lanes : Anti-VPS11 antibody (ab125083) at 0.04 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : Jurkat whole cell lysate at 50 µg
    Lane 4 : HeLa whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 108 kDa

    Exposure time: 3 minutes
  • Detection of VPS11 by Western Blot of Immunprecipitate.
    ab125083 at 0.4µg/ml staining VPS11 in 293T whole cell lysate immunoprecipitated using ab125083 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.


This product has been referenced in:

  • Marwaha R  et al. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes. J Cell Biol 216:1051-1070 (2017). Read more (PubMed: 28325809) »
  • Sindhwani A  et al. Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication. PLoS Pathog 13:e1006700 (2017). Read more (PubMed: 29084291) »
See all 3 Publications for this product

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