Product nameAnti-VPS11 antibody
See all VPS11 primary antibodies
DescriptionRabbit polyclonal to VPS11
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide, corresponding to a region within amino acids 891-941 of Human VPS11 (NP_068375.3).
- 293T, Jurkat and HeLa whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab125083 was affinity purified using an epitope specific to VPS11 immobilized on solid support.
Our Abpromise guarantee covers the use of ab125083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 108 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
Tissue specificityUbiquitous. Expression was highest in heart and low in lung.
Sequence similaritiesBelongs to the VPS11 family.
Contains 1 clathrin repeat.
Contains 1 RING-type zinc finger.
Cellular localizationLate endosome membrane. Lysosome membrane. Cytoplasmic vesicle.
- Information by UniProt
- END1 antibody
- HGNC:14583 antibody
- hVPS11 antibody
All lanes : Anti-VPS11 antibody (ab125083) at 0.04 µg/ml
Lane 1 : 293T whole cell lysate at 50 µg
Lane 2 : 293T whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 50 µg
Lane 4 : HeLa whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 108 kDa
Exposure time: 3 minutes
Detection of VPS11 by Western Blot of Immunprecipitate.
ab125083 at 0.4µg/ml staining VPS11 in 293T whole cell lysate immunoprecipitated using ab125083 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
This product has been referenced in:
- Marwaha R et al. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes. J Cell Biol 216:1051-1070 (2017). Read more (PubMed: 28325809) »
- Sindhwani A et al. Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication. PLoS Pathog 13:e1006700 (2017). Read more (PubMed: 29084291) »