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  1. Link

    vps11-antibody-epr10345-ab170869.pdf

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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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Recombinant
RabMAb

Recombinant Anti-VPS11 antibody [EPR10345] (ab170869)

  • Datasheet
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  • Western blot - Anti-VPS11 antibody [EPR10345] (ab170869)
  • Immunoprecipitation - Anti-VPS11 antibody [EPR10345] (ab170869)
  • Immunocytochemistry/ Immunofluorescence - Anti-VPS11 antibody [EPR10345] (ab170869)

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  • Datasheet
  • References (1)
  • Protocols

Overview

  • Product name

    Anti-VPS11 antibody [EPR10345]
    See all VPS11 primary antibodies
  • Description

    Rabbit monoclonal [EPR10345] to VPS11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VPS11 aa 900 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9H270

  • Positive control

    • Human fetal brain, K562, 293T and HeLa lysates, HeLa cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10345
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation

Associated products

  • Alternative Versions

    • Anti-VPS11 antibody [EPR10345] - BSA and Azide free (ab249551)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • K562 whole cell lysate (ab7911)
    • 293T whole cell lysate (ab95494)
  • Recombinant Protein

    • Recombinant Human VPS11 protein (ab132470)

Applications

Our Abpromise guarantee covers the use of ab170869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 108 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
    • Tissue specificity

      Ubiquitous. Expression was highest in heart and low in lung.
    • Sequence similarities

      Belongs to the VPS11 family.
      Contains 1 clathrin repeat.
      Contains 1 RING-type zinc finger.
    • Cellular localization

      Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle.
    • Target information above from: UniProt accession Q9H270 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 55823 Human
      • Entrez Gene: 71732 Mouse
      • Entrez Gene: 315600 Rat
      • Omim: 608549 Human
      • SwissProt: Q9H270 Human
      • SwissProt: Q91W86 Mouse
      • Unigene: 234282 Human
      • Unigene: 295013 Mouse
      • Unigene: 2637 Rat
      see all
    • Alternative names

      • END1 antibody
      • HGNC:14583 antibody
      • hVPS11 antibody
      • PEP5 antibody
      • PP3476 antibody
      • RING finger protein 108 antibody
      • RNF108 antibody
      • Vacuolar protein sorting 11 (yeast homolog) antibody
      • Vacuolar protein sorting 11 (yeast) antibody
      • Vacuolar protein sorting 11 homolog (S. cerevisiae) antibody
      • Vacuolar protein sorting 11 homolog antibody
      • Vacuolar protein sorting associated protein 11 homolog antibody
      • Vacuolar protein sorting protein 11 antibody
      • Vacuolar protein sorting-associated protein 11 homolog antibody
      • vps11 antibody
      • VPS11_HUMAN antibody
      see all

    Images

    • Western blot - Anti-VPS11 antibody [EPR10345] (ab170869)
      Western blot - Anti-VPS11 antibody [EPR10345] (ab170869)
      All lanes : Anti-VPS11 antibody [EPR10345] (ab170869) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 108 kDa

    • Immunoprecipitation - Anti-VPS11 antibody [EPR10345] (ab170869)
      Immunoprecipitation - Anti-VPS11 antibody [EPR10345] (ab170869)
      Anti-VPS11 antibody [EPR10345] (ab170869) at 1/10 dilution + 293T cell lysate
    • Immunocytochemistry/ Immunofluorescence - Anti-VPS11 antibody [EPR10345] (ab170869)
      Immunocytochemistry/ Immunofluorescence - Anti-VPS11 antibody [EPR10345] (ab170869)

      Immunofluorescent staining of HeLa cells labeling VPS11 using ab170869 at a 1/100 dilution (red) and DAPI nuclear staining (blue).

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols

    Datasheets and documents

    • Datasheet
  • References

    This product has been referenced in:

    • Chandra S  et al. Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae. Nat Commun 9:4279 (2018). Read more (PubMed: 30323255) »
    See 1 Publication for this product

    Publishing research using ab170869? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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