Recombinant
RabMAb

Recombinant Anti-VPS11 antibody [EPR10345] (ab170869)

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Overview

  • Product name

    Anti-VPS11 antibody [EPR10345]
    See all VPS11 primary antibodies

  • Description

    Rabbit monoclonal [EPR10345] to VPS11

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VPS11 aa 900 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9H270

  • Positive control

    • Human fetal brain, K562, 293T and HeLa lysates, HeLa cells

  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10345

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 108 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

    • Tissue specificity

      Ubiquitous. Expression was highest in heart and low in lung.

    • Sequence similarities

      Belongs to the VPS11 family.
      Contains 1 clathrin repeat.
      Contains 1 RING-type zinc finger.

    • Cellular localization

      Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • END1 antibody
      • HGNC:14583 antibody
      • hVPS11 antibody
      • PEP5 antibody
      • PP3476 antibody
      • RING finger protein 108 antibody
      • RNF108 antibody
      • Vacuolar protein sorting 11 (yeast homolog) antibody
      • Vacuolar protein sorting 11 (yeast) antibody
      • Vacuolar protein sorting 11 homolog (S. cerevisiae) antibody
      • Vacuolar protein sorting 11 homolog antibody
      • Vacuolar protein sorting associated protein 11 homolog antibody
      • Vacuolar protein sorting protein 11 antibody
      • Vacuolar protein sorting-associated protein 11 homolog antibody
      • vps11 antibody
      • VPS11_HUMAN antibody
      see all

    Images

    • Western blot - Anti-VPS11 antibody [EPR10345] (ab170869)
      Western blot - Anti-VPS11 antibody [EPR10345] (ab170869)
      All lanes : Anti-VPS11 antibody [EPR10345] (ab170869) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 108 kDa

    • Immunoprecipitation - Anti-VPS11 antibody [EPR10345] (ab170869)
      Immunoprecipitation - Anti-VPS11 antibody [EPR10345] (ab170869)
      Anti-VPS11 antibody [EPR10345] (ab170869) at 1/10 dilution + 293T cell lysate
    • Immunocytochemistry/ Immunofluorescence - Anti-VPS11 antibody [EPR10345] (ab170869)
      Immunocytochemistry/ Immunofluorescence - Anti-VPS11 antibody [EPR10345] (ab170869)

      Immunofluorescent staining of HeLa cells labeling VPS11 using ab170869 at a 1/100 dilution (red) and DAPI nuclear staining (blue).

    References

    This product has been referenced in:

    • Chandra S  et al. Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae. Nat Commun 9:4279 (2018). Read more (PubMed: 30323255) »
    See 1 Publication for this product

    Publishing research using ab170869? Please let us know so that we can cite the reference in this datasheet.

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