RecombinantRabMAb

Recombinant Anti-VPS11 antibody [EPR10345] (ab170869)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10345] to VPS11
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-VPS11 antibody [EPR10345]
    See all VPS11 primary antibodies

  • Description

    Rabbit monoclonal [EPR10345] to VPS11

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VPS11 aa 900 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9H270

  • Positive control

    • Human fetal brain, K562, 293T and HeLa lysates, HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR10345

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 108 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

    • Tissue specificity

      Ubiquitous. Expression was highest in heart and low in lung.

    • Sequence similarities

      Belongs to the VPS11 family.
      Contains 1 clathrin repeat.
      Contains 1 RING-type zinc finger.

    • Cellular localization

      Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • END1 antibody
      • HGNC:14583 antibody
      • hVPS11 antibody
      • PEP5 antibody
      • PP3476 antibody
      • RING finger protein 108 antibody
      • RNF108 antibody
      • Vacuolar protein sorting 11 (yeast homolog) antibody
      • Vacuolar protein sorting 11 (yeast) antibody
      • Vacuolar protein sorting 11 homolog (S. cerevisiae) antibody
      • Vacuolar protein sorting 11 homolog antibody
      • Vacuolar protein sorting associated protein 11 homolog antibody
      • Vacuolar protein sorting protein 11 antibody
      • Vacuolar protein sorting-associated protein 11 homolog antibody
      • vps11 antibody
      • VPS11_HUMAN antibody
      see all

    Images

    • Western blot - Anti-VPS11 antibody [EPR10345] (ab170869)
      Western blot - Anti-VPS11 antibody [EPR10345] (ab170869)
      All lanes : Anti-VPS11 antibody [EPR10345] (ab170869) at 1/1000 dilution

      Lane 1 : Human fetal brain lysate
      Lane 2 : K562 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 108 kDa

    • Immunoprecipitation - Anti-VPS11 antibody [EPR10345] (ab170869)
      Immunoprecipitation - Anti-VPS11 antibody [EPR10345] (ab170869)
      Anti-VPS11 antibody [EPR10345] (ab170869) at 1/10 dilution + HEK-293T cell lysate
    • Immunocytochemistry/ Immunofluorescence - Anti-VPS11 antibody [EPR10345] (ab170869)
      Immunocytochemistry/ Immunofluorescence - Anti-VPS11 antibody [EPR10345] (ab170869)

      Immunofluorescent staining of HeLa cells labeling VPS11 using ab170869 at a 1/100 dilution (red) and DAPI nuclear staining (blue).

    • Anti-VPS11 antibody [EPR10345] (ab170869)
      Anti-VPS11 antibody [EPR10345] (ab170869)

    References (1)

    Publishing research using ab170869? Please let us know so that we can cite the reference in this datasheet.

    ab170869 has been referenced in 1 publication.

    • Chandra S  et al. Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae. Nat Commun 9:4279 (2018). PubMed: 30323255

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