
Recombinant Anti-VPS11 antibody [EPR10345] (ab170869)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-VPS11 antibody [EPR10345]
See all VPS11 primary antibodies -
Description
Rabbit monoclonal [EPR10345] to VPS11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VPS11 aa 900 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9H270 -
Positive control
- Human fetal brain, K562, 293T and HeLa lysates, HeLa cells
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10345 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab170869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Predicted molecular weight: 108 kDa. | |
ICC/IF | 1/100 - 1/250. | |
IP | 1/10 - 1/100. |
Target
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Function
May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. -
Tissue specificity
Ubiquitous. Expression was highest in heart and low in lung. -
Sequence similarities
Belongs to the VPS11 family.
Contains 1 clathrin repeat.
Contains 1 RING-type zinc finger. -
Cellular localization
Late endosome membrane. Lysosome membrane. Cytoplasmic vesicle. - Information by UniProt
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Database links
- Entrez Gene: 55823 Human
- Entrez Gene: 71732 Mouse
- Entrez Gene: 315600 Rat
- Omim: 608549 Human
- SwissProt: Q9H270 Human
- SwissProt: Q91W86 Mouse
- Unigene: 234282 Human
- Unigene: 295013 Mouse
see all -
Alternative names
- END1 antibody
- HGNC:14583 antibody
- hVPS11 antibody
see all
Images
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All lanes : Anti-VPS11 antibody [EPR10345] (ab170869) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : K562 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 108 kDa -
Anti-VPS11 antibody [EPR10345] (ab170869) at 1/10 dilution + 293T cell lysate
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Immunofluorescent staining of HeLa cells labeling VPS11 using ab170869 at a 1/100 dilution (red) and DAPI nuclear staining (blue).
Protocols
Datasheets and documents
References
This product has been referenced in:
- Chandra S et al. Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae. Nat Commun 9:4279 (2018). Read more (PubMed: 30323255) »