Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10345] to VPS11
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Product nameAnti-VPS11 antibody [EPR10345]
See all VPS11 primary antibodies
DescriptionRabbit monoclonal [EPR10345] to VPS11
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VPS11 aa 900 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9H270
- Human fetal brain, K562, 293T and HeLa lysates, HeLa cells
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab170869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 108 kDa.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
FunctionMay play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
Tissue specificityUbiquitous. Expression was highest in heart and low in lung.
Sequence similaritiesBelongs to the VPS11 family.
Contains 1 clathrin repeat.
Contains 1 RING-type zinc finger.
Cellular localizationLate endosome membrane. Lysosome membrane. Cytoplasmic vesicle.
- Information by UniProt
- END1 antibody
- HGNC:14583 antibody
- hVPS11 antibody
All lanes : Anti-VPS11 antibody [EPR10345] (ab170869) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : K562 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 108 kDa
Anti-VPS11 antibody [EPR10345] (ab170869) at 1/10 dilution + HEK-293T cell lysate
Immunofluorescent staining of HeLa cells labeling VPS11 using ab170869 at a 1/100 dilution (red) and DAPI nuclear staining (blue).
ab170869 has been referenced in 1 publication.
- Chandra S et al. Mrp1 is involved in lipid presentation and iNKT cell activation by Streptococcus pneumoniae. Nat Commun 9:4279 (2018). PubMed: 30323255