Anti-VPS13B/CHS1 antibody (ab139814)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-VPS13B/CHS1 antibody -
Description
Rabbit polyclonal to VPS13B/CHS1 -
Host species
Rabbit -
Specificity
At least five alternatively spliced transcript variants have been observed. ab139814 detects two isoforms. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human VPS13B/CHS1 (N terminal).
Database link: NP_056058 -
Positive control
- SK-N-SH cell lysate.
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General notes
This product was previously labelled as VPS13B
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab139814 was purified by affinity chromatography via a peptide column. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab139814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 20 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 97, 159 kDa. |
Target
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Function
May be involved in protein sorting in post Golgi membrane traffic. -
Tissue specificity
Widely expressed. There is apparent differential expression of different transcripts. In fetal brain, lung, liver, and kidney, two transcripts of 2 and 5 kb are identified. These transcripts are also seen in all adult tissues analyzed. A larger transcript (12-14 kb) is expressed in prostate, testis, ovary, and colon in the adult. Expression is very low in adult brain tissue. Isoform 1 and isoform 2 are expressed in brain and retina. Isoform 2 is expressed ubiquitously. -
Involvement in disease
Defects in VPS13B are a cause of Cohen syndrome (COH1) [MIM:216550]. COH1 is a rare autosomal recessive disorder characterized by obesity, hypotonia, intellectual deficit, characteristic craniofacial dysmorphism and abnormalities of the hands and feet. Characteristic facial features include high-arched or wave-shaped eyelids, a short philtrum, thick hair and low hairline. -
Sequence similarities
Belongs to the VPS13 family. - Information by UniProt
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Database links
- Entrez Gene: 157680 Human
- Entrez Gene: 666173 Mouse
- Entrez Gene: 315036 Rat
- Omim: 607817 Human
- SwissProt: Q7Z7G8 Human
- SwissProt: Q80TY5 Mouse
- Unigene: 191540 Human
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Alternative names
- CHS1 antibody
- COH1 antibody
- Cohen syndrome protein 1 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-VPS13B/CHS1 antibody (ab139814)Immunofluorescence of VPS13B/CHS1 in Human brain tissue with ab139814 at 20 ug/mL.
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Western blot - Anti-VPS13B/CHS1 antibody (ab139814)Lane 1 : Anti-VPS13B/CHS1 antibody (ab139814) at 1 µg/ml
Lane 2 : Anti-VPS13B/CHS1 antibody (ab139814) at 2 µg/ml
All lanes : SK-N-SH cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 97, 159 kDa
Datasheets and documents
References
ab139814 has not yet been referenced specifically in any publications.
