Key features and details
- Rabbit polyclonal to VPS13D
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-VPS13D antibody
See all VPS13D primary antibodies
DescriptionRabbit polyclonal to VPS13D
Tested applicationsSuitable for: IPmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
Synthetic peptide within Human VPS13D aa 2150-2200. The exact sequence is proprietary. NP_056193.2
Database link: Q5THJ4
- Whole cell lysate from Jurkat cells
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab202285 was affinity purified using an epitope specific to VPS13D immobilized on solid support.
Our Abpromise guarantee covers the use of ab202285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the VPS13 family.
Contains 1 UBA domain.
- Information by UniProt
- KIAA0453 antibody
- vacuolar protein sorting 13 homolog D (S. cerevisiae) antibody
- vacuolar protein sorting 13D antibody
Lane 1: Detection of Human VPS13D by Immunoprecipitation from Jurkat whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using ab202285 at 6 µg per reaction for IP.
Lane 2: Immunoprecipitation from Jurkat whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using an antibody which recognized an epitope of VPS13D.
Lane 3: Immunoprecipitation from Jurkat whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using Control IgG.
Subsequent Western blot detection used ab202285 at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3
ab202285 has not yet been referenced specifically in any publications.