Overview

  • Product name
  • Description
    Rabbit polyclonal to VPS13D
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human VPS13D aa 2150-2200. The exact sequence is proprietary. NP_056193.2
    Database link: Q5THJ4

  • Positive control
    • Whole cell lysate from Jurkat cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab202285 was affinity purified using an epitope specific to VPS13D immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab202285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    Images

    • Lane 1: Detection of Human VPS13D by Immunoprecipitation from Jurkat whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using ab202285 at 6 µg per reaction for IP.

      Lane 2: Immunoprecipitation from Jurkat whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using an antibody which recognized an epitope of VPS13D.
      Lane 3: Immunoprecipitation from Jurkat whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using Control IgG.
      Subsequent Western blot detection used ab202285 at 1 µg/ml.

       

      Detection: Chemiluminescence with an exposure time of 3
      minutes.

    References

    ab202285 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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