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Rabbit polyclonal to VPS13D
Predicted to work with:
Rabbit, Horse, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
Synthetic peptide within Human VPS13D aa 2150-2200. The exact sequence is proprietary. NP_056193.2
Database link: Q5THJ4
Whole cell lysate from Jurkat cells
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab202285 was affinity purified using an epitope specific to VPS13D immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
Is unsuitable for WB.
Belongs to the VPS13 family.
Contains 1 UBA domain.
Information by UniProt
vacuolar protein sorting 13 homolog D (S. cerevisiae) antibody
vacuolar protein sorting 13D antibody
Immunoprecipitation - Anti-VPS13D antibody (ab202285)
Lane 1: Detection of Human VPS13D by Immunoprecipitation from Jurkat whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using ab202285 at 6 µg per reaction for IP.
Lane 2: Immunoprecipitation from Jurkat whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using an antibody which recognized an epitope of VPS13D.
Lane 3: Immunoprecipitation from Jurkat whole cell lysate (0.5 to 1 mg for IP, 20% of IP loaded), using Control IgG. Subsequent Western blot detection used ab202285 at 1 µg/ml.
Detection: Chemiluminescence with an exposure time of 3
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"