Overview

  • Product name

  • Description

    Rabbit polyclonal to VPS15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human VPS15 aa 1314-1325 (C terminal).
    Sequence:

    DDTPRRGPESLP


    Database link: Q99570

  • Positive control

    • ICC/IF: SK-N-BE cells. WB: Rat brain lysate.
  • General notes

     This product was previously labelled as PI 3 Kinase regulatory subunit 4

     

Properties

Applications

Our Abpromise guarantee covers the use of ab240914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB 1/1000. Predicted molecular weight: 153 kDa.

Target

  • Function

    Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 3 HEAT repeats.
    Contains 1 protein kinase domain.
    Contains 7 WD repeats.
  • Post-translational
    modifications

    Myristoylated.
    Probably autophosphorylated.
  • Information by UniProt
  • Database links

  • Alternative names

    • MGC102700 antibody
    • P150 antibody
    • Phosphatidylinositol 3 kinase associated p150 antibody
    • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4 antibody
    • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4 p150 antibody
    • Phosphoinositide 3 kinase adaptor protein antibody
    • Phosphoinositide 3 kinase regulatory subunit 4 antibody
    • Phosphoinositide 3 kinase regulatory subunit 4 p150 antibody
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 4 antibody
    • Phosphoinositide 3 kinase regulatory subunit polypeptide 4 p150 antibody
    • Phosphoinositide 3-kinase adaptor protein antibody
    • Phosphoinositide 3-kinase regulatory subunit 4 antibody
    • PI3 kinase p150 subunit antibody
    • PI3-kinase p150 subunit antibody
    • PI3-kinase regulatory subunit 4 antibody
    • PI3R4_HUMAN antibody
    • PIK3R 4 antibody
    • PIK3R4 antibody
    • VPS 15 antibody
    • VPS15 antibody
    see all

Images

  • 4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells labeling VPS15 (green) using ab240914 at 1/100 dilution in ICC/IF, followed by a goat anti-rabbit ATTO 488 conjugated secondary antibody at 1/200 dilution. Both primary and secondary antibodies were incubated for 1 hour at RT. Counterstained with Phalloidin Texas Red F-Actin stain (red) at 1/1000 for 1 hour at RT. The nuclear counterstain in DAPI (blue) at 1/5000 for 5 minutes at RT.

    (A) DAPI (blue) nuclear stain

    (B) Phalloidin Texas Red F-Actin stain (red)

    (C) ab240914

    (D) Composite

  • Anti-VPS15 antibody (ab240914) at 1/1000 dilution + Rat brain lysate at 15 µg

    Secondary
    HRP-linked goat anti-rabbit IgG at 1/2000 dilution

    Developed using the ECL technique.

    Predicted band size: 153 kDa


    Exposure time: 6 minutes


    Blocked with 5% skim milk in 1XTBST.

    Primary and secondary incubation: 1 hour at RT.

References

ab240914 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab240914.
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