Key features and details
- Rabbit polyclonal to VPS15
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Product nameAnti-VPS15 antibody
See all VPS15 primary antibodies
DescriptionRabbit polyclonal to VPS15
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse, Orangutan
This product was previously labelled as PI 3 Kinase regulatory subunit 4
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab240914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 153 kDa.|
FunctionRegulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 3 HEAT repeats.
Contains 1 protein kinase domain.
Contains 7 WD repeats.
- Information by UniProt
- MGC102700 antibody
- P150 antibody
- Phosphatidylinositol 3 kinase associated p150 antibody
4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells labeling VPS15 (green) using ab240914 at 1/100 dilution in ICC/IF, followed by a goat anti-rabbit ATTO 488 conjugated secondary antibody at 1/200 dilution. Both primary and secondary antibodies were incubated for 1 hour at RT. Counterstained with Phalloidin Texas Red F-Actin stain (red) at 1/1000 for 1 hour at RT. The nuclear counterstain in DAPI (blue) at 1/5000 for 5 minutes at RT.
(A) DAPI (blue) nuclear stain
(B) Phalloidin Texas Red F-Actin stain (red)
Anti-VPS15 antibody (ab240914) at 1/1000 dilution + Rat brain lysate at 15 µg
HRP-linked goat anti-rabbit IgG at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 153 kDa
Exposure time: 6 minutes
Blocked with 5% skim milk in 1XTBST.
Primary and secondary incubation: 1 hour at RT.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab240914 has not yet been referenced specifically in any publications.