Overview

  • Product name

    Anti-VPS15 antibody [EPR5302(2)]
    See all VPS15 primary antibodies
  • Description

    Rabbit monoclonal [EPR5302(2)] to VPS15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VPS15 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Jurkat, 293T, HeLa, Raji, and MOLT4 cell lysates; 293T cells.
  • General notes

     

     

     This product was previously labelled as PI 3 Kinase regulatory subunit 4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128903 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa).
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function

      Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 3 HEAT repeats.
      Contains 1 protein kinase domain.
      Contains 7 WD repeats.
    • Post-translational
      modifications

      Myristoylated.
      Probably autophosphorylated.
    • Information by UniProt
    • Database links

    • Alternative names

      • MGC102700 antibody
      • P150 antibody
      • Phosphatidylinositol 3 kinase associated p150 antibody
      • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4 antibody
      • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4 p150 antibody
      • Phosphoinositide 3 kinase adaptor protein antibody
      • Phosphoinositide 3 kinase regulatory subunit 4 antibody
      • Phosphoinositide 3 kinase regulatory subunit 4 p150 antibody
      • Phosphoinositide 3 kinase regulatory subunit polypeptide 4 antibody
      • Phosphoinositide 3 kinase regulatory subunit polypeptide 4 p150 antibody
      • Phosphoinositide 3-kinase adaptor protein antibody
      • Phosphoinositide 3-kinase regulatory subunit 4 antibody
      • PI3 kinase p150 subunit antibody
      • PI3-kinase p150 subunit antibody
      • PI3-kinase regulatory subunit 4 antibody
      • PI3R4_HUMAN antibody
      • PIK3R 4 antibody
      • PIK3R4 antibody
      • VPS 15 antibody
      • VPS15 antibody
      see all

    Images

    • All lanes : Anti-VPS15 antibody [EPR5302(2)] (ab128903) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : Raji cell lysate
      Lane 5 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 153 kDa

    • ab128903 at 1/50 dilution staining VPS15 in 293T cells by Immunofluorescence.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Gstrein T  et al. Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans. Nat Neurosci 21:207-217 (2018). Read more (PubMed: 29311744) »
    • Wang M  et al. WDR81 regulates adult hippocampal neurogenesis through endosomal SARA-TGFß signaling. Mol Psychiatry N/A:N/A (2018). Read more (PubMed: 30531936) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    HEK cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Aug 08 2019

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monkey Cell (Kidney)
    Permeabilization
    Yes - 0.1% troton x-100
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jun 14 2016

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3% Triton x 100: 30 min
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Apr 05 2016

    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (Kidney)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    Kidney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Apr 05 2016

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Apr 05 2016

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