Recombinant Anti-VPS15 antibody [EPR5302(2)] (ab128903)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5302(2)] to VPS15
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-VPS15 antibody [EPR5302(2)]
See all VPS15 primary antibodies -
Description
Rabbit monoclonal [EPR5302(2)] to VPS15 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human VPS15 aa 50-150. The exact sequence is proprietary.
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Positive control
- WB: Raji, C6, and Neuro-2a whole cell lysates. Mouse 14.5 brain, Rat E14 brain, and Rat P0 brain lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 1.22 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5302(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa).
For unpurified use at 1/1000 - 1/10000. |
Notes |
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WB
1/500. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa). For unpurified use at 1/1000 - 1/10000. |
Target
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Function
Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 3 HEAT repeats.
Contains 1 protein kinase domain.
Contains 7 WD repeats. -
Post-translational
modificationsMyristoylated.
Probably autophosphorylated. - Information by UniProt
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Database links
- Entrez Gene: 30849 Human
- Entrez Gene: 75669 Mouse
- Entrez Gene: 363131 Rat
- Omim: 602610 Human
- SwissProt: Q99570 Human
- SwissProt: Q8VD65 Mouse
- SwissProt: P0C0R5 Rat
- Unigene: 149032 Human
see all -
Alternative names
- MGC102700 antibody
- P150 antibody
- Phosphatidylinositol 3 kinase associated p150 antibody
see all
Images
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Anti-VPS15 antibody [EPR5302(2)] (ab128903) at 1/1000 dilution (Purified) + Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 153 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This antibody detects high background.
Blocking/Diluting buffer: 5% NFDM/TBST
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All lanes : Anti-VPS15 antibody [EPR5302(2)] (ab128903) at 1/500 dilution (Purified)
Lane 1 : Mouse E14.5 brain lysates
Lane 2 : Rat E14 brain lysates
Lane 3 : Rat P0 brain lysates
Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 5 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 153 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?Blocking/Diluting buffer: 5% NFDM/TBST
This antibody detects high background.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab128903 has been referenced in 6 publications.
- Liu Y et al. Phosphoinositide-3-kinase regulatory subunit 4 participates in the occurrence and development of amyotrophic lateral sclerosis by regulating autophagy. Neural Regen Res 17:1609-1616 (2022). PubMed: 34916448
- Wrobel L et al. Compounds activating VCP D1 ATPase enhance both autophagic and proteasomal neurotoxic protein clearance. Nat Commun 13:4146 (2022). PubMed: 35842429
- Gillingham AK et al. In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation. Elife 8:N/A (2019). PubMed: 31294692
- Sharoar MG et al. Sequential formation of different layers of dystrophic neurites in Alzheimer's brains. Mol Psychiatry N/A:N/A (2019). PubMed: 30899091
- Gstrein T et al. Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans. Nat Neurosci 21:207-217 (2018). PubMed: 29311744
- Wang M et al. WDR81 regulates adult hippocampal neurogenesis through endosomal SARA-TGFß signaling. Mol Psychiatry N/A:N/A (2018). PubMed: 30531936