Recombinant Anti-VPS15 antibody [EPR5302(2)] - BSA and Azide free (ab248210)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5302(2)] to VPS15 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-VPS15 antibody [EPR5302(2)] - BSA and Azide free
See all VPS15 primary antibodies -
Description
Rabbit monoclonal [EPR5302(2)] to VPS15 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Raji, C6, and Neuro-2a whole cell lysates. Mouse 14.5 brain, Rat E14 brain, and Rat P0 brain lysates.
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General notes
ab248210 is the carrier-free version of ab128903.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.22 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5302(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa). |
Target
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Function
Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 3 HEAT repeats.
Contains 1 protein kinase domain.
Contains 7 WD repeats. -
Post-translational
modificationsMyristoylated.
Probably autophosphorylated. - Information by UniProt
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Database links
- Entrez Gene: 30849 Human
- Entrez Gene: 75669 Mouse
- Entrez Gene: 363131 Rat
- Omim: 602610 Human
- SwissProt: Q99570 Human
- SwissProt: Q8VD65 Mouse
- SwissProt: P0C0R5 Rat
- Unigene: 149032 Human
see all -
Alternative names
- MGC102700 antibody
- P150 antibody
- Phosphatidylinositol 3 kinase associated p150 antibody
see all
Images
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Anti-VPS15 antibody [EPR5302(2)] (ab128903) at 1/1000 dilution (Purified) + Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 153 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This antibody detects high background.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128903).
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All lanes : Anti-VPS15 antibody [EPR5302(2)] (ab128903) at 1/500 dilution (Purified)
Lane 1 : Mouse E14.5 brain lysates
Lane 2 : Rat E14 brain lysates
Lane 3 : Rat P0 brain lysates
Lane 4 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 5 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 153 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?This antibody detects high background.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab128903).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab248210 has not yet been referenced specifically in any publications.