Product nameAnti-VPS15 antibody [EPR5303(2)]
See all VPS15 primary antibodies
DescriptionRabbit monoclonal [EPR5303(2)] to VPS15
Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Rat, Human
- Raji cell lysate, Jurkat cell lysate, Molt-4 cell lysate, 293T cell lysate, C6 cell lysate and Raw 264.7 cell lysate. Human kidney tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab124817 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa).Can be blocked with VPS15 peptide (ab183696).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionRegulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 3 HEAT repeats.
Contains 1 protein kinase domain.
Contains 7 WD repeats.
- Information by UniProt
- MGC102700 antibody
- P150 antibody
- Phosphatidylinositol 3 kinase associated p150 antibody
All lanes : Anti-VPS15 antibody [EPR5303(2)] (ab124817) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Molt-4 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : C6 cell lysate
Lane 6 : Raw 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 153 kDa
ab124817 at 1/100 dilution, staining VPS15 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.