Overview

  • Product name

    Anti-VPS15 antibody [EPR5303(2)]
    See all VPS15 primary antibodies
  • Description

    Rabbit monoclonal [EPR5303(2)] to VPS15
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VPS15 aa 1300 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q99570
    (Peptide available as ab183696)

  • Positive control

    • Raji cell lysate, Jurkat cell lysate, Molt-4 cell lysate, 293T cell lysate, C6 cell lysate and Raw 264.7 cell lysate. Human kidney tissue.
  • General notes

     

     

     This product was previously labelled as PI 3 Kinase regulatory subunit 4

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab124817 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 153 kDa).Can be blocked with VPS15 peptide (ab183696).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Regulatory subunit of the PI3K complex. May regulate membrane trafficking late in the endocytic pathway.
    • Tissue specificity

      Ubiquitously expressed.
    • Sequence similarities

      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
      Contains 3 HEAT repeats.
      Contains 1 protein kinase domain.
      Contains 7 WD repeats.
    • Post-translational
      modifications

      Myristoylated.
      Probably autophosphorylated.
    • Information by UniProt
    • Database links

    • Alternative names

      • MGC102700 antibody
      • P150 antibody
      • Phosphatidylinositol 3 kinase associated p150 antibody
      • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4 antibody
      • Phosphatidylinositol 3 kinase regulatory subunit polypeptide 4 p150 antibody
      • Phosphoinositide 3 kinase adaptor protein antibody
      • Phosphoinositide 3 kinase regulatory subunit 4 antibody
      • Phosphoinositide 3 kinase regulatory subunit 4 p150 antibody
      • Phosphoinositide 3 kinase regulatory subunit polypeptide 4 antibody
      • Phosphoinositide 3 kinase regulatory subunit polypeptide 4 p150 antibody
      • Phosphoinositide 3-kinase adaptor protein antibody
      • Phosphoinositide 3-kinase regulatory subunit 4 antibody
      • PI3 kinase p150 subunit antibody
      • PI3-kinase p150 subunit antibody
      • PI3-kinase regulatory subunit 4 antibody
      • PI3R4_HUMAN antibody
      • PIK3R 4 antibody
      • PIK3R4 antibody
      • VPS 15 antibody
      • VPS15 antibody
      see all

    Images

    • All lanes : Anti-VPS15 antibody [EPR5303(2)] (ab124817) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Molt-4 cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : Raw 264.7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 153 kDa

    • ab124817 at 1/100 dilution, staining VPS15 in Formalin fixed Paraffin-embedded Human kidney tissue by Immunohistochemistry.

    References

    This product has been referenced in:

    • Gstrein T  et al. Mutations in Vps15 perturb neuronal migration in mice and are associated with neurodevelopmental disease in humans. Nat Neurosci 21:207-217 (2018). Read more (PubMed: 29311744) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124817.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up