Overview

  • Product name

  • Description

    Goat polyclonal to VPS16
  • Host species

    Goat
  • Specificity

    ab206323 is expected to recognize both reported isoforms (NP_072097.2; NP_536338.1).
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human VPS16 aa 490-502 (internal sequence). NP_072097.2; NP_536338.1.
    Sequence:

    CYKVQQKDVSDED


    Database link: Q9H269

  • Positive control

    • HepG2 cell line lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab206323 was purified from goat serum by ammonium sulfate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 80,95 kDa (predicted molecular weight: 95 kDa).

Target

  • Function

    May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the VPS16 family.
  • Cellular localization

    Late endosome membrane. Lysosome membrane. Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • HGNC:14584 antibody
    • hVPS16 antibody
    • mVPS16 antibody
    • RP11 12M19.2 antibody
    • Vacuolar protein sorting 16 (yeast) antibody
    • Vacuolar protein sorting 16 (Yeast), isoform CRA_a antibody
    • Vacuolar protein sorting 16 antibody
    • Vacuolar protein sorting 16 homolog (S. cerevisiae) antibody
    • vacuolar protein sorting associated protein 16 homolog antibody
    • Vacuolar protein sorting protein 16 antibody
    • Vacuolar protein sorting-associated protein 16 homolog antibody
    • VPS16 antibody
    • VPS16_HUMAN antibody
    • VPS16A antibody
    see all

Images

  • Anti-VPS16 antibody (ab206323) at 1 µg/ml + HepG2 cell line lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 95 kDa



    Primary antibody incuabtion was 1 hour.

  • Anti-VPS16 antibody (ab206323) at 0.5 µg/ml + Mouse spleen tissue lysate at 35 µg

    Predicted band size: 95 kDa
    Observed band size: 95 kDa



    Primary incubation was 1 hour. Detected by chemiluminescence.

References

ab206323 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab206323.
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