Overview

  • Product name

  • Description

    Rabbit polyclonal to VPS18
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human VPS18 aa 1-50. The exact sequence is proprietary.
    Database link: Q9P253

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: VPS18 IP in HEK-293T whole cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab240574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
  • Tissue specificity

    Ubiquitous. Expression was highest in heart and low in lung.
  • Sequence similarities

    Belongs to the VPS18 family.
    Contains 1 clathrin repeat.
    Contains 1 RING-type zinc finger.
  • Cellular localization

    Late endosome membrane. Lysosome membrane. Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • hVPS18 antibody
    • PEP3 antibody
    • Vacuolar protein sorting 18 antibody
    • Vacuolar protein sorting protein 18 antibody
    • Vacuolar protein sorting-associated protein 18 homolog antibody
    • vps18 antibody
    • VPS18_HUMAN antibody
    see all

Images

  • All lanes : Anti-VPS18 antibody (ab240574) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Exposure time: 30 seconds
  • HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
    Lane 1: ab240574 used for IP at 6 µg per reaction. For WB 1 µg/ml.
    Lane 2: Control IgG.
    Chemiluminescence detection: 3 minutes.

References

ab240574 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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