Product nameAnti-VPS18 antibody
See all VPS18 primary antibodies
DescriptionRabbit polyclonal to VPS18
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human VPS18 aa 1-50. The exact sequence is proprietary.
Database link: Q9P253
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysate. IP: VPS18 IP in HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab240574 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
Tissue specificityUbiquitous. Expression was highest in heart and low in lung.
Sequence similaritiesBelongs to the VPS18 family.
Contains 1 clathrin repeat.
Contains 1 RING-type zinc finger.
Cellular localizationLate endosome membrane. Lysosome membrane. Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.
- Information by UniProt
- hVPS18 antibody
- PEP3 antibody
- Vacuolar protein sorting 18 antibody
All lanes : Anti-VPS18 antibody (ab240574) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 seconds
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
Lane 1: ab240574 used for IP at 6 µg per reaction. For WB 1 µg/ml.
Lane 2: Control IgG.
Chemiluminescence detection: 3 minutes.
ab240574 has not yet been referenced specifically in any publications.