Key features and details
- Rabbit polyclonal to VPS18
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-VPS18 antibody
See all VPS18 primary antibodies
DescriptionRabbit polyclonal to VPS18
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Synthetic peptide within Human VPS18 aa 900-950. The exact sequence is proprietary.
Database link: Q9P253
- WB: HeLa, HEK-293T and Jurkat cell lysate. IP: HEK-293T cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab241131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000.|
FunctionMay play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
Tissue specificityUbiquitous. Expression was highest in heart and low in lung.
Sequence similaritiesBelongs to the VPS18 family.
Contains 1 clathrin repeat.
Contains 1 RING-type zinc finger.
Cellular localizationLate endosome membrane. Lysosome membrane. Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.
- Information by UniProt
- hVPS18 antibody
- PEP3 antibody
- Vacuolar protein sorting 18 antibody
All lanes : Anti-VPS18 antibody (ab241131) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
Prepared using NETN lysis buffer.
VPS18 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241131 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241131 at 1 µg/ml.
Lane 1: ab241131 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241131 has not yet been referenced specifically in any publications.