Overview

  • Product name

    Anti-VPS18 antibody - C-terminal
    See all VPS18 primary antibodies
  • Description

    Rabbit polyclonal to VPS18 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human VPS18 (C terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
    Database link: Q9P253

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. ICC/IF: A431 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab229335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 110 kDa.

Target

  • Function

    May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
  • Tissue specificity

    Ubiquitous. Expression was highest in heart and low in lung.
  • Sequence similarities

    Belongs to the VPS18 family.
    Contains 1 clathrin repeat.
    Contains 1 RING-type zinc finger.
  • Cellular localization

    Late endosome membrane. Lysosome membrane. Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.
  • Information by UniProt
  • Database links

  • Alternative names

    • hVPS18 antibody
    • PEP3 antibody
    • Vacuolar protein sorting 18 antibody
    • Vacuolar protein sorting protein 18 antibody
    • Vacuolar protein sorting-associated protein 18 homolog antibody
    • vps18 antibody
    • VPS18_HUMAN antibody
    see all

Images

  • All lanes : Anti-VPS18 antibody - C-terminal (ab229335) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 110 kDa



    7.5% SDS-PAGE gel.

  • A431 (human epidermoid carcinoma cell line) cells stained for VPS18 (green) using ab229335 at 1/1000 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229335 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229335.
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