Recombinant
RabMAb

Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416)

Overview

  • Product name
    Anti-VPS18 antibody [EPR13378] - N-terminal
    See all VPS18 primary antibodies
  • Description
    Rabbit monoclonal [EPR13378] to VPS18 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VPS18 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9P253

  • Positive control
    • HeLa, 293T and K562 cell lysates; 293T cells; K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR13378
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab178416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
    • Tissue specificity
      Ubiquitous. Expression was highest in heart and low in lung.
    • Sequence similarities
      Belongs to the VPS18 family.
      Contains 1 clathrin repeat.
      Contains 1 RING-type zinc finger.
    • Cellular localization
      Late endosome membrane. Lysosome membrane. Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • hVPS18 antibody
      • PEP3 antibody
      • Vacuolar protein sorting 18 antibody
      • Vacuolar protein sorting protein 18 antibody
      • Vacuolar protein sorting-associated protein 18 homolog antibody
      • vps18 antibody
      • VPS18_HUMAN antibody
      see all

    Images

    • All lanes : Anti-VPS18 antibody [EPR13378] - N-terminal (ab178416) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 110 kDa
      Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.

    • Immunofluorescent analysis of 293T cells labeling VPS18 with ab178416 at 1/100 (green) and DAPI staining (blue).

    • Flow Cytometrical analysis of permeabilized K562 cells labeling VPS18 with ab178416 at 1/10 (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:
    • Marwaha R  et al. The Rab7 effector PLEKHM1 binds Arl8b to promote cargo traffic to lysosomes. J Cell Biol 216:1051-1070 (2017). Read more (PubMed: 28325809) »
    • Sindhwani A  et al. Salmonella exploits the host endolysosomal tethering factor HOPS complex to promote its intravacuolar replication. PLoS Pathog 13:e1006700 (2017). Read more (PubMed: 29084291) »

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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