Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13379] to VPS18
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
Product nameAnti-VPS18 antibody [EPR13379]
See all VPS18 primary antibodies
DescriptionRabbit monoclonal [EPR13379] to VPS18
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VPS18 aa 950 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9P253
- HeLa, A549, 293T and K562 cell lysates. A549 cells. Permeabilized 293T cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab178689 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 110 kDa.|
|Flow Cyt||1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/100 - 1/250.|
FunctionMay play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
Tissue specificityUbiquitous. Expression was highest in heart and low in lung.
Sequence similaritiesBelongs to the VPS18 family.
Contains 1 clathrin repeat.
Contains 1 RING-type zinc finger.
Cellular localizationLate endosome membrane. Lysosome membrane. Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.
- Information by UniProt
- hVPS18 antibody
- PEP3 antibody
- Vacuolar protein sorting 18 antibody
All lanes : Anti-VPS18 antibody [EPR13379] (ab178689) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : A549 cell lysates
Lane 3 : 293T cell lysates
Lane 4 : K562 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 110 kDa
Immunofluorescence analysis of A549 cells labeling VPS18 with ab178689 at a 1/100 dilution. DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized 293T cells using ab178689 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).
ab178689 has not yet been referenced specifically in any publications.