Overview

  • Product name

    Anti-VPS18 antibody [EPR13379]
    See all VPS18 primary antibodies
  • Description

    Rabbit monoclonal [EPR13379] to VPS18
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human VPS18 aa 950 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9P253

  • Positive control

    • HeLa, A549, 293T and K562 cell lysates. A549 cells. Permeabilized 293T cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13379
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab178689 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 110 kDa.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      May play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.
    • Tissue specificity

      Ubiquitous. Expression was highest in heart and low in lung.
    • Sequence similarities

      Belongs to the VPS18 family.
      Contains 1 clathrin repeat.
      Contains 1 RING-type zinc finger.
    • Cellular localization

      Late endosome membrane. Lysosome membrane. Cytoplasmic, peripheral membrane protein associated with late endosomes/lysosomes.
    • Information by UniProt
    • Database links

    • Alternative names

      • hVPS18 antibody
      • PEP3 antibody
      • Vacuolar protein sorting 18 antibody
      • Vacuolar protein sorting protein 18 antibody
      • Vacuolar protein sorting-associated protein 18 homolog antibody
      • vps18 antibody
      • VPS18_HUMAN antibody
      see all

    Images

    • All lanes : Anti-VPS18 antibody [EPR13379] (ab178689) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : A549 cell lysates
      Lane 3 : 293T cell lysates
      Lane 4 : K562 cell lysates

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 110 kDa

    • Immunofluorescence analysis of A549 cells labeling VPS18 with ab178689 at a 1/100 dilution. DAPI nuclear staining (blue).

    • Flow cytometric analysis of permeabilized 293T cells using ab178689 at a 1/100 dilution (red) or a rabbit IgG (negative) (green).

    References

    ab178689 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab178689.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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