Overview

  • Product name
    Anti-VPS24 antibody [EPR12821]
  • Description
    Rabbit monoclonal [EPR12821] to VPS24
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IP, Flow Cytmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human VPS24. The exact sequence is proprietary.
    Database link: Q9Y3E7

  • Positive control
    • 293T, HepG2, U87-MG, fetal brain lysates; human cervical carcinoma tissue, human prostate tissue, 293T cells
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12821
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Selectively binds to phosphatidylinositol 3,5-bisphosphate PtdIns(3,5)P2 and PtdIns(3,4)P2 in preference to other phosphoinositides tested. Involved in late stages of cytokinesis. Required for sorting/trafficking of EGF receptor.
    • Tissue specificity
      Widely expressed. Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
    • Sequence similarities
      Belongs to the SNF7 family.
    • Domain
      The acidic C-terminus and the basic N-termminus are thought to render the protein in a closed, soluble and inactive conformation through an autoinhibitory intramolecular interaction. The open and active conformation, which enables membrane binding and oligomerization, is achieved by interaction with other cellular binding partners, probably including other ESCRT components.
    • Cellular localization
      Cytoplasm > cytosol. Membrane. Endosome. Late endosome membrane. Localizes to the midbody of dividing cells.
    • Information by UniProt
    • Database links
    • Alternative names
      • VPS 24 antibody
      • VPS24 antibody
      • CGI 149 antibody
      • CGI149 protein antibody
      • Charged multivesicular body protein 3 antibody
      • CHMP3 antibody
      • CHMP3_HUMAN antibody
      • Chromatin modifying protein 3 antibody
      • Chromatin-modifying protein 3 antibody
      • HGNC:29865 antibody
      • hVps24 antibody
      • NEDF antibody
      • Neuroendocrine differentiation factor antibody
      • Vacuolar protein sorting 24 (yeast) antibody
      • Vacuolar protein sorting 24 homolog (S. cerevisiae) antibody
      • Vacuolar protein sorting associated protein 24 antibody
      • Vacuolar protein sorting-associated protein 24 antibody
      • vps24 antibody
      see all

    Images

    • All lanes : Anti-VPS24 antibody [EPR12821] (ab175930) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : U87-MG cell lysate
      Lane 4 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 29 kDa

    • Immunohistochemical analysis of paraffin-embedded human prostate tissue labeling VPS24 with ab175930 at 1/50 dilution

    • Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue using labeling VPS24 with ab175930 at 1/50 dilution

    • Western blot analysis on immunoprecipitation pellet from (1) HepG2 cells lysate or (2) 1X PBS (negative control) using ab175930 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    • Flow cytometric analysis of permeabilized 293T cells  labeling VPS24 with ab175930 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    ab175930 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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